Ródenas V, García M S, Sánchez-Pedreño C, Albero M I
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain.
J Pharm Biomed Anal. 1997 Jul;15(11):1687-93. doi: 10.1016/s0731-7085(96)01963-2.
Sensitive and fast spectrophotometric methods for the determination of cephradine or ceftazidine in human urine, based on the formation of compounds between these drugs and Pd(II), are described. In the batch procedures the calibration graphs resulting from the measurement of the absorbance at 330 nm is linear over the range 5.0-60.0 micrograms. ml-1 for cephradine and 3.0-60.0 micrograms ml-1 for ceftazidine. The methods were successfully adapted to FI-systems, the peak heights being proportional to cephalosporin concentration over the range 5.0-60.0 micrograms ml-1 for cephradine and 3.0-60.0 micrograms ml-1 for ceftazidine. The sampling frequency was 60 h-1 with a sample injection of 72 microliters.
本文描述了基于头孢拉定或头孢他啶与钯（II）形成化合物来测定人尿中头孢拉定或头孢他啶的灵敏快速分光光度法。在分批程序中，在330nm处测量吸光度得到的校准曲线对于头孢拉定在5.0 - 60.0微克·毫升⁻¹范围内呈线性，对于头孢他啶在3.0 - 60.0微克·毫升⁻¹范围内呈线性。这些方法成功应用于流动注射系统，峰高与头孢菌素浓度成正比，对于头孢拉定在5.0 - 60.0微克·毫升⁻¹范围内，对于头孢他啶在3.0 - 60.0微克·毫升⁻¹范围内。进样量为72微升时，采样频率为60次/小时。
