Ikeda I, Kasajima T, Ishiyama S, Shimojo T, Takeo Y, Nishikawa T, Kameoka S, Hiroe M, Mitsunaga A
Department of Pathology, Tokyo Women's Medical College, Shinjuku-ku, Japan.
Am J Gastroenterol. 1997 Aug;92(8):1339-41.
To investigate reports that the biosynthesis of nitric oxide (NO) is increased in the colonic mucosa of patients with active ulcerative colitis (UC), which suggests that serum NOx levels may be an important indicator of UC activity.
To determine the role of NO within the colon, we purchased polyclonal antibodies against human-inducible NO synthase (iNOS). We then examined the distribution of iNOS-reactive cells in UC colon tissues.
In specimens from 12 UC patients, iNOS-positive neutrophils and macrophages were observed at the base of the ulcer but not in distant areas in the active stage. iNOS expression in colon mucosa was virtually absent during the inactive stage of UC and within the colon of patients with non-UC colitis.
We conclude that NO in colonic mucosa may play a potential role in the pathogenesis of UC.
调查关于活动性溃疡性结肠炎（UC）患者结肠黏膜中一氧化氮（NO）生物合成增加的报道，这表明血清NOx水平可能是UC活动的重要指标。
为确定NO在结肠中的作用，我们购买了抗人诱导型一氧化氮合酶（iNOS）的多克隆抗体。然后我们检查了iNOS反应性细胞在UC结肠组织中的分布。
在12例UC患者的标本中，在溃疡底部观察到iNOS阳性的中性粒细胞和巨噬细胞，但在活动期的远处区域未观察到。在UC的非活动期以及非UC结肠炎患者的结肠中，结肠黏膜中几乎不存在iNOS表达。
我们得出结论，结肠黏膜中的NO可能在UC的发病机制中起潜在作用。
