Rachmilewitz D, Eliakim R, Ackerman Z, Karmeli F
Department of Medicine, Hadassah University Hospital, Mount Scopus, Hebrew University Hadassah Medical School, Jerusalem, Israel.
Am J Gastroenterol. 1998 Mar;93(3):409-12. doi: 10.1111/j.1572-0241.1998.00409.x.
In active ulcerative colitis, colonic nitric oxide (NO) generation is enhanced and probably has an important role in its pathogenesis. We tested the reliability of an NO electrode in monitoring colonic NO levels in ulcerative colitis patients and control subjects and its possible usage as a marker of disease activity.
Colonic NO level was determined by the NO detection system model NO-501 (InterMedical, Nagoya, Japan). The working electrode was inserted into a 7-mm diameter polyvinyl tube and introduced at a distance 6 cm from the anus. In each subject sigmoidoscopy was performed and mucosal biopsies were obtained. NO synthase (NOS) activity was determined by monitoring the conversion of 3H-arginine to citrulline.
Colonic NO level is significantly increased in patients with active ulcerative or Crohn's colitis--more than 2-fold higher than in control subjects. There was good correlation between colonic NO level and NOS activity and the clinical and endoscopic indices of disease activity.
Direct determination of colonic NO level is convenient, and reliable, and may help to monitor disease activity in ulcerative colitis.
在活动性溃疡性结肠炎中，结肠一氧化氮（NO）的生成增加，其可能在发病机制中起重要作用。我们测试了一种NO电极监测溃疡性结肠炎患者和对照受试者结肠NO水平的可靠性，以及其作为疾病活动标志物的潜在用途。
采用NO-501型NO检测系统（日本名古屋InterMedical公司）测定结肠NO水平。将工作电极插入直径7mm的聚乙烯管中，并从肛门6cm处插入。对每个受试者进行乙状结肠镜检查并获取黏膜活检标本。通过监测3H-精氨酸向瓜氨酸的转化来测定一氧化氮合酶（NOS）活性。
活动性溃疡性结肠炎或克罗恩病患者的结肠NO水平显著升高，比对照受试者高2倍以上。结肠NO水平与NOS活性以及疾病活动的临床和内镜指标之间存在良好的相关性。
直接测定结肠NO水平简便、可靠，可能有助于监测溃疡性结肠炎的疾病活动。
