Persson E, Rodriguez-Martinez H
Department of Anatomy and Histology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
Microsc Res Tech. 1997;38(1-2):165-75. doi: 10.1002/(SICI)1097-0029(19970701/15)38:1/2<165::AID-JEMT17>3.0.CO;2-N.
The immunocytochemical localization of insulin-like growth factor I (IGF-I) and platelet-derived growth factor A (PDGF-A) chain ligands, PDGF-chain receptors, and the intermediate filaments cytokeratin, desmin, and vimentin in the tissue reorganization and development during the establishment of the porcine epitheliochorial placenta was studied at light and electron microscopic levels in sections of endometrium and/or placenta of gilts during early pregnancy up to day 40 postmating. The endometrial epithelia (surface and glandular) as well as the trophoblast showed PDGF-A and IGF-I labelling. The only temporal difference was seen regarding IGF-I labelling, this being more uniform in the glands during precontact and early contact stages (days 7-12) compared to later stages. Cytokeratin labelling was conspicuous in all epithelia, including the trophoblast. The endometrial stroma showed strong labelling for the PDGF receptors and the intermediate filament vimentin, staining being enhanced along with the increase in the vascular bed during the establishment of the placenta. The maternal endothelium immunoreacted to IGF-I, to both PDGF-A and PDGF receptors, to vimentin and to von Willebrand factor (Factor VIII). Desmin was also expressed in the capillary bed underneath the maternal surface epithelium. In larger blood vessels, desmin was detected in the smooth muscle layer but not in the endothelium. The vascular smooth muscle also reacted with IGF-I, PDGF-A, and PDGF-receptor antibodies. The results suggest the involvement of both growth factors, IGF-I and PDGF, in the early nutrition of the pig embryo and the tissue reorganization that encompasses placentation. IGF-I appears to be related to the secretory tissue compartment (formation of histotrophe), whereas PDGF might play a role in the reorganization of the stroma, particularly during placental angiogenesis. Desmin and von Willebrand factor immunolabelling appears to be useful to monitor the development of the subepithelial capillary bed in the porcine placenta.
在妊娠早期直至配种后40天的后备母猪子宫内膜和/或胎盘切片中，利用光镜和电镜技术，研究了胰岛素样生长因子I（IGF-I）、血小板衍生生长因子A（PDGF-A）链配体、PDGF链受体以及中间丝细胞角蛋白、结蛋白和波形蛋白在猪上皮绒毛膜胎盘建立过程中的组织重组和发育中的免疫细胞化学定位。子宫内膜上皮（表面和腺上皮）以及滋养层均显示出PDGF-A和IGF-I标记。关于IGF-I标记，仅观察到时间上的差异，与后期相比，在接触前和早期接触阶段（第7 - 12天）腺体中的标记更为均匀。细胞角蛋白标记在所有上皮中都很明显，包括滋养层。子宫内膜基质对PDGF受体和中间丝波形蛋白显示出强烈标记，随着胎盘建立过程中血管床的增加，染色增强。母体内皮对IGF-I、PDGF-A和PDGF受体、波形蛋白以及血管性血友病因子（因子VIII）有免疫反应。结蛋白也在母体表面上皮下方的毛细血管床中表达。在较大的血管中，结蛋白在平滑肌层中被检测到，但在内皮中未检测到。血管平滑肌也与IGF-I、PDGF-A和PDGF受体抗体发生反应。结果表明，生长因子IGF-I和PDGF均参与了猪胚胎的早期营养以及包括胎盘形成在内的组织重组。IGF-I似乎与分泌组织区室（组织营养物的形成）有关，而PDGF可能在基质重组中发挥作用，特别是在胎盘血管生成过程中。结蛋白和血管性血友病因子免疫标记似乎有助于监测猪胎盘上皮下毛细血管床的发育。
