酵母Ty逆转录转座子病毒样颗粒的冷冻电子显微镜结构
Cryo-electron microscopy structure of yeast Ty retrotransposon virus-like particles.
作者信息
Palmer K J, Tichelaar W, Myers N, Burns N R, Butcher S J, Kingsman A J, Fuller S D, Saibil H R
机构信息
Department of Crystallography, Birkbeck College, London, United Kingdom.
出版信息
J Virol. 1997 Sep;71(9):6863-8. doi: 10.1128/JVI.71.9.6863-6868.1997.
Abstract
The virus-like particles (VLPs) produced by the yeast retrotransposon Ty1 are functionally related to retroviral cores. These particles are unusual in that they have variable radif. A paired mass-radius analysis of VLPs by scanning transmission electron microscopy showed that many of these particles form an icosahedral T-number series. Three-dimensional reconstruction to 38-A resolution from cryo-electron micrographs of T = 3 and T = 4 shells revealed that the single structural protein encoded by the TYA gene assembles into spiky shells from trimeric units.
摘要
酵母逆转录转座子Ty1产生的病毒样颗粒（VLP）在功能上与逆转录病毒核心相关。这些颗粒不同寻常之处在于它们具有可变半径。通过扫描透射电子显微镜对VLP进行的配对质量-半径分析表明，许多这些颗粒形成二十面体T数系列。从T = 3和T = 4壳层的冷冻电子显微照片进行三维重建至38埃分辨率，结果显示由TYA基因编码的单一结构蛋白从三聚体单元组装成带刺的壳层。
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