Horie S, Nakada K, Masuyama J, Yoshio T, Minota S, Wakabayashi Y, Hirose S, Kano S
Department of Medicine, Saiseikai Hospital, Utsunomiya, Japan.
J Rheumatol. 1997 Aug;24(8):1517-21.
To test for the presence of colony forming cells, that form large macrophage colonies (> 2.5 mm in diameter, > 10,000 cells), in the peripheral blood of patients with rheumatoid arthritis (RA) and to determine its association with the clinical and laboratory features of RA.
Peripheral blood mononuclear cells (PBMC) from 96 patients with RA and 20 healthy controls were assayed for in vitro colony formation. In addition, PBMC from 38 patients with other rheumatic diseases including systemic lupus erythematosus (SLE), progressive systemic sclerosis (SSc), and polymyositis/dermatomyositis (PM/DM); 23 patients with infectious inflammatory diseases were also assayed.
Large macrophage colony forming cells were detected in the peripheral blood of 19% of patients with RA (18/96), but not in that of healthy controls. In addition, these cells were detected in the peripheral blood of 11 of the 38 patients with other rheumatic disease (7/13 SSc and 4/11 PM/DM), but not in the 23 patients with infectious diseases. In the patients with RA, interstitial lung disease was significantly more frequently observed among patients in whom colony forming cells were found than among those in whom they were not found (p < 0.001).
Based on the size of the colonies they formed, the macrophage colony forming cells detected in patients with RA probably corresponded to primitive hematopoietic progenitor cells, defined as high proliferative potential colony forming cells (HPP-CFC). Our observations provide preliminary evidence of the appearance of HPP-CFC in the circulation during inflammation of RA, and during that in other rheumatic diseases such as SSc and PM/DM, and of the association of HPP-CFC with interstitial lung disease in patients with RA.
检测类风湿关节炎（RA）患者外周血中形成大型巨噬细胞集落（直径>2.5毫米，细胞数>10,000个）的集落形成细胞的存在情况，并确定其与RA临床及实验室特征的关联。
对96例RA患者和20例健康对照者的外周血单个核细胞（PBMC）进行体外集落形成检测。此外，还对38例其他风湿性疾病患者（包括系统性红斑狼疮（SLE）、进行性系统性硬化症（SSc）和多发性肌炎/皮肌炎（PM/DM））以及23例感染性炎症疾病患者的PBMC进行了检测。
19%的RA患者（18/96）外周血中检测到大型巨噬细胞集落形成细胞，而健康对照者中未检测到。此外，38例其他风湿性疾病患者中有11例（7/13 SSc和4/11 PM/DM）外周血中检测到这些细胞，而23例感染性疾病患者中未检测到。在RA患者中，发现集落形成细胞的患者间质性肺疾病的发生率显著高于未发现集落形成细胞的患者（p<0.001）。
根据RA患者中检测到的巨噬细胞集落形成细胞所形成集落的大小，这些细胞可能对应于原始造血祖细胞，即高增殖潜能集落形成细胞（HPP-CFC）。我们的观察结果为RA炎症期间以及其他风湿性疾病（如SSc和PM/DM）炎症期间循环中出现HPP-CFC以及RA患者中HPP-CFC与间质性肺疾病的关联提供了初步证据。
