Davey H M, Kell D B
Institute of Biological Sciences, University of Wales, Aberystwyth, Dyfed, United Kingdom.
Cytometry. 1997 Aug 1;28(4):311-5. doi: 10.1002/(sici)1097-0320(19970801)28:4<311::aid-cyto6>3.0.co;2-e.
Flow cytometry is a rapid method for measuring the optical properties of individual cells. The technique has found great utility in the study of mammalian cells, but microbiological applications have been more limited. We here show that UV-excited fluorescent whitening agents, in particular Tinopal CBS-X, are effective stains for both vegetative microbial cells and for spores of Gram-positive bacteria. Pretreatment of samples with ethanol speeds the staining process. Under favourable conditions, Tinopal CBS-X may be used to discriminate among organisms, a fact that may be useful when screening for a target microorganism against a high biological background.
流式细胞术是一种用于测量单个细胞光学特性的快速方法。该技术在哺乳动物细胞研究中已得到广泛应用，但在微生物学中的应用则较为有限。我们在此表明，紫外线激发的荧光增白剂，特别是Tinopal CBS-X，对营养型微生物细胞和革兰氏阳性菌的孢子都是有效的染色剂。用乙醇预处理样品可加快染色过程。在有利条件下，Tinopal CBS-X可用于区分不同的生物体，这一事实在针对高生物背景筛选目标微生物时可能会很有用。
