Hipp M L, Bauer G
Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, Germany.
Oncogene. 1997 Aug 14;15(7):791-7. doi: 10.1038/sj.onc.1201247.
Transformed fibroblast from p53 null/null mice were tested for their sensitivity to intercellular induction of apoptosis by TGF-beta-treated nontransformed cells. They were found to be as sensitive as p53-positive transformed cells. Based on morphological criteria, detection of chromatin condensation and DNA strand breaks, death of p53-negative transformed cells was due to apoptosis. p53-negative nontransformed cells were as efficient in the induction of apoptosis in transformed cells as p53-positive nontransformed cells. These data show that intercellular induction of apoptosis in transformed cells does not depend on functional p53. Therefore it may be assumed that mutations of p53 or modulation of its concentration are without relevance for this particular aspect of control of oncogenesis.
对来自p53基因敲除小鼠的转化成纤维细胞进行测试，以检测其对经TGF-β处理的未转化细胞诱导细胞间凋亡的敏感性。结果发现它们与p53阳性转化细胞一样敏感。根据形态学标准、染色质浓缩和DNA链断裂的检测，p53阴性转化细胞的死亡是由于凋亡。p53阴性未转化细胞在诱导转化细胞凋亡方面与p53阳性未转化细胞一样有效。这些数据表明，转化细胞中细胞间凋亡的诱导不依赖于功能性p53。因此，可以假设p53的突变或其浓度的调节与肿瘤发生控制的这一特定方面无关。
