Rice F L, Fundin B T, Arvidsson J, Aldskogius H, Johansson O
Department of Pharmacology and Neuroscience Albany Medical College, New York 12054, USA.
J Comp Neurol. 1997 Aug 25;385(2):149-84.
The innervation of the vibrissal follicle sinus complexes (FSCs) in the mystacial pad of the rat was examined by lectin binding histofluorescence with the B subunit of Griffonia simplicifolia (GSA) and by immunofluorescence with a wide variety of antibodies for neuronal related structural proteins, enzymes, and peptides. Only anti-protein gene product 9.5 labeled all sets of innervation. Several types of mechanoreceptors were distributed to specific different targets by medium to large caliber myelinated axons. All were positive for 200 kDa neurofilament subunit, peripherin, and carbonic anhydrase. Their endings expressed synaptophysin. Labeling for the 160 kDa neurofilament subunit, calbindin, and parvalbumin varied. Anti-Schwann cell protein S100 was completely co-extensive with the axons, terminal arbors, and endings of the mechanoreceptor afferents including Merkel innervation. At least 15 different sets of unmyelinated innervation were evident based upon distribution and labeling characteristics. They consisted of four basic types: 1) peptidergic; 2) GSA binding; 3) peptidergic and GSA binding; and 4) nonpeptidergic and GSA negative (peptide-/GSA-). Previous studies had not revealed that several major sets of unmyelinated innervation were peptide-/GSA-. The unmyelinated innervation had detectable peripherin but not 160 kDa or 200 kDa neurofilament subunits. GSA-positive axons uniquely lacked anti-S100 immunoreactivity. The dense circumferentially oriented unmyelinated innervation of the inner conical body contained major sets of peptide-/GSA- and GSA innervation as well as a smaller peptidergic GSA component. A small contingent of sympathetic and possibly parasympathetic innervation was affiliated with microvasculature in the FSCs. This study confirms and refutes some previous hypotheses about biochemical and morphological relationships between peripheral innervation and sensory ganglion cells.
采用伴刀豆球蛋白A（GSA）的B亚基进行凝集素结合组织荧光法，以及使用多种针对神经元相关结构蛋白、酶和肽的抗体进行免疫荧光法，研究大鼠触须垫中触须毛囊窦复合体（FSCs）的神经支配情况。只有抗蛋白基因产物9.5标记了所有神经支配组。几种类型的机械感受器由中到大口径的有髓轴突分布到特定的不同靶标。所有这些感受器对200 kDa神经丝亚基、外周蛋白和碳酸酐酶均呈阳性。它们的末梢表达突触素。160 kDa神经丝亚基、钙结合蛋白和小白蛋白的标记情况各不相同。抗施万细胞蛋白S100与包括默克尔神经支配在内的机械感受器传入神经的轴突、终末分支和末梢完全共定位。根据分布和标记特征，至少有15种不同的无髓神经支配组是明显的。它们由四种基本类型组成：1）肽能的；2）GSA结合的；3）肽能且GSA结合的；4）非肽能且GSA阴性（肽-/GSA-）。先前的研究并未揭示几种主要的无髓神经支配组是肽-/GSA-。无髓神经支配有可检测到的外周蛋白，但没有160 kDa或200 kDa神经丝亚基。GSA阳性轴突独特地缺乏抗S100免疫反应性。内锥体密集的圆周定向无髓神经支配包含主要的肽-/GSA-和GSA神经支配组以及较小的肽能GSA成分。一小部分交感神经和可能的副交感神经支配与FSCs中的微血管相关。本研究证实并反驳了一些先前关于外周神经支配与感觉神经节细胞之间生化和形态关系的假设。
