Green S, Kurane I, Pincus S, Paoletti E, Ennis F A
Department of Medicine, University of Massachusetts Medical Center, Worcester 01655, USA.
Virology. 1997 Aug 4;234(2):383-6. doi: 10.1006/viro.1997.8648.
We examined nine dengue virus-specific human CD4+ CD8- cytotoxic T lymphocyte (CTL) clones for protein recognition, using recombinant vaccinia viruses which contain genes coding for dengue virus proteins. These clones were established from peripheral blood mononuclear cells of a donor previously immunized with a live-attenuated experimental dengue-1 vaccine. Of nine CD4+ T cell clones, seven were dengue-1-specific and two were dengue-1-dengue-3 cross-reactive. Four dengue-1-specific clones and one dengue-1-dengue-3 cross-reactive clone recognized epitopes within the NS1 or NS2a proteins. Analysis of human leukocyte antigen (HLA) restriction revealed that three dengue-1-specific clones are HLA-DR1-restricted and one dengue-1-dengue-3 cross-reactive clone is HLA-DPw3-restricted. These results indicate that NS1 and NS2a proteins as well as C, E, and NS3 proteins reported earlier contain one or more epitopes recognized by dengue virus-specific human CD4+ T lymphocytes.
我们使用含有登革病毒蛋白编码基因的重组痘苗病毒，检测了9个登革病毒特异性人类CD4+ CD8-细胞毒性T淋巴细胞（CTL）克隆的蛋白识别情况。这些克隆是从一名先前接种过减毒活实验性登革1型疫苗的供体的外周血单个核细胞中建立的。在9个CD4+ T细胞克隆中，7个是登革1型特异性的，2个是登革1型-登革3型交叉反应性的。4个登革1型特异性克隆和1个登革1型-登革3型交叉反应性克隆识别NS1或NS2a蛋白内的表位。人类白细胞抗原（HLA）限制性分析显示，3个登革1型特异性克隆受HLA-DR1限制，1个登革1型-登革3型交叉反应性克隆受HLA-DPw3限制。这些结果表明，NS1和NS2a蛋白以及先前报道的C、E和NS3蛋白含有一个或多个被登革病毒特异性人类CD4+ T淋巴细胞识别的表位。
