Kut C, Hornebeck W, Groult N, Redziniack G, Godeau G, Pellat B
Biochemistry Department, Faculty of Dental Surgery, University René Descartes Paris V, 1 rue M. Arnoux, Montrouge, 92120, France.
Cell Biol Int. 1997 Jun;21(6):347-52. doi: 10.1006/cbir.1997.0150.
To study the cumulative influence of UV irradiations on skin matrix alterations, human skin fibroblasts were irradiated successively three-fold, at 24 h intervals, with UVA (3x5J/cm2), UVB (3x8mJ/cm2), UVA plus UVB (3x5J/cm2 and 3x8mJ/cm2) and the levels of 92 kDa gelatinase (pro-MMP9), 72 kDa gelatinase (pro-MMP2) and plasma-membrane elastase type protease were determined, following subsequent 24-h culture in 10% serum-containing medium. UV irradiations had only minor influence (1.4-fold increase for UVB) on secreted levels of pro-MMP2 and decreased the amount of plasma membrane elastase produced by cells. It did however, for UVA and UVB alone, induce a significant increase of 66 kDa activated MMP2 production: 2.5- and 1.7-fold respectively. Such enhancement was not observed when combined irradiations were administered. UV exposure possessed a much higher influence on pro-MMP9 secretion by dermal fibroblast enhancing enzyme levels by 2.5-, 6.5- and 5-fold for UVA, UVB and UVA+UVB, respectively.
为研究紫外线照射对皮肤基质改变的累积影响，以24小时间隔对人皮肤成纤维细胞进行三次连续照射，分别采用UVA（3×5J/cm2）、UVB（3×8mJ/cm2）、UVA加UVB（3×5J/cm2和3×8mJ/cm2）照射，然后在含10%血清的培养基中培养24小时后，测定92kDa明胶酶（前MMP9）、72kDa明胶酶（前MMP2）和质膜弹性蛋白酶型蛋白酶的水平。紫外线照射对前MMP2的分泌水平影响较小（UVB照射后增加1.4倍），并减少细胞产生的质膜弹性蛋白酶量。然而，单独的UVA和UVB照射确实诱导了66kDa活化MMP2产生的显著增加：分别为2.5倍和1.7倍。当进行联合照射时未观察到这种增强作用。紫外线暴露对真皮成纤维细胞分泌前MMP9的影响大得多，UVA、UVB和UVA + UVB分别使酶水平提高2.5倍、6.5倍和5倍。
