Triviño A, Ramírez J M, Ramírez A I, Salazar J J, García-Sanchez J
Instituto de Investigaciones Oftalmológicas Ramón Castroviejo, Facultad de Medicina, Universidad Complutense, Madrid, Spain.
Vision Res. 1997 Jul;37(13):1707-11. doi: 10.1016/s0042-6989(97)00021-7.
Immunohistochemical location of Glial Fibrillary Acidic Protein (GFAP) was used to compare the morphology of astrocytes in vascularized and partially vascularized retinae (human and rabbit, respectively). Astrocytes in human and rabbit retinae were found in the same regions as the blood vessels. These cells in partially vascularized retinae differed from those in vascularized retina in several respects. Firstly, there were six morphological types in rabbit retina and only two in human retina. Secondly, in rabbit retinae there were astrocytes only related to blood vessels called "perivascular astrocytes" which were absent in human retinae. Thirdly, the astrocytes were located in the nerve fiber layer and ganglion cell layer in both types of retinae, but in human retinae these cells could also be seen in the internal nuclear layer. These observations demonstrate that there are many differences between astrocytes in human and rabbit retina, suggesting that rabbit retina could be used with caution as an experimental model in comparative studies with human retina.
采用胶质纤维酸性蛋白（GFAP）免疫组化定位法，比较血管化视网膜和部分血管化视网膜（分别为人类和兔视网膜）中星形胶质细胞的形态。人类和兔视网膜中的星形胶质细胞与血管分布在相同区域。部分血管化视网膜中的这些细胞在几个方面与血管化视网膜中的细胞不同。首先，兔视网膜中有六种形态类型，而人类视网膜中只有两种。其次，兔视网膜中存在仅与血管相关的星形胶质细胞，称为“血管周围星形胶质细胞”，而人类视网膜中不存在。第三，两种视网膜类型中的星形胶质细胞均位于神经纤维层和神经节细胞层，但在人类视网膜中，这些细胞也可见于内核层。这些观察结果表明，人类和兔视网膜中的星形胶质细胞存在许多差异，这表明在与人类视网膜的比较研究中，兔视网膜作为实验模型应谨慎使用。
