Al-Bader A A, Mosawi M H, Hussain T A, Dashti H M
Department of Pathology, Faculty of Medicine, Kuwait University Health Sciences Center, Safat.
Mol Cell Biochem. 1997 Aug;173(1-2):121-5. doi: 10.1023/a:1006835912530.
The effect of thioacetamide-induced liver cirrhosis on plasma and tissue manganese levels and the protective role of selenium, zinc and allopurinol supplements was investigated in rats. Control plasma and liver manganese (Mn) levels were found to be (mean +/- SD): 8.4 +/- 2.4 mg/L and 5.7 +/- 1.5 mg/g wet weight respectively. Plasma manganese levels were significantly increased (p < 0.001) whereas liver manganese levels were significantly reduced (p < 0.05) in the cirrhotic rats. Treatment with selenium, zinc and allopurinol reversed this trend and restored the manganese levels close to the normal values. Lung, spleen, and kidney manganese levels under control conditions were considerably lower than that of the liver tissue. However, these levels registered a significant increase (p < 0.05) in cirrhotic rats and this change was normalized after selenium, zinc and allopurinol treatment. There were no significant differences in the comparative efficacy of each of these protective agents. Zinc supplement considerably increased the plasma zinc levels and plasma Zn/Mn ratio had a good correlation with plasma zinc concentration. This ratio was significantly reduced in cirrhotic rats, but returned to the control level after zinc, selenium and allopurinol treatment. The results of this study indicate that the trace element, manganese, plays an important role in stabilizing cell structure and that this effect is mediated possibly by preserving the antioxidant activity of the tissues.
在大鼠中研究了硫代乙酰胺诱导的肝硬化对血浆和组织锰水平的影响以及硒、锌和别嘌呤醇补充剂的保护作用。发现对照大鼠的血浆和肝脏锰（Mn）水平分别为（平均值±标准差）：8.4±2.4mg/L和5.7±1.5mg/g湿重。肝硬化大鼠的血浆锰水平显著升高（p<0.001），而肝脏锰水平显著降低（p<0.05）。用硒、锌和别嘌呤醇治疗可逆转这一趋势，并使锰水平恢复至接近正常值。对照条件下肺、脾和肾的锰水平明显低于肝脏组织。然而，这些水平在肝硬化大鼠中显著升高（p<0.05），而在硒、锌和别嘌呤醇治疗后这一变化恢复正常。这些保护剂各自的比较疗效无显著差异。补充锌可显著提高血浆锌水平，血浆锌/锰比值与血浆锌浓度具有良好的相关性。该比值在肝硬化大鼠中显著降低，但在锌、硒和别嘌呤醇治疗后恢复至对照水平。本研究结果表明，微量元素锰在稳定细胞结构中起重要作用，且这种作用可能通过维持组织的抗氧化活性来介导。
