Palumbo B, Parnetti L, Nocentini G, Cardinali L, Brancorsini S, Riccardi C, Senin U
Department of Clinical Medicine, Pathology and Pharmacology, University of Perugia, Italy.
Neurosci Lett. 1997 Aug 1;231(1):59-61. doi: 10.1016/s0304-3940(97)00538-7.
Apolipoprotein-E (Apo-E) genotype and allele frequencies were evaluated in patients with late-onset probable Alzheimer's disease (LOAD; n = 64), early-onset probable Alzheimer's disease (EOAD; n = 32), possible Alzheimer's disease (pAD; n = 44), vascular dementia (VD; n = 12), age-associated memory impairment (AAMI; n = 15) and 40 healthy age-matched controls. APO-E was performed by polymerase chain reaction products digested by the restriction enzyme HhaI. A statistically significant increase of epsilon4 frequency was found in LOAD as compared to the other groups, and in pAD with respect to controls, while VD and AAMI groups did not disclose any difference as regards to control subjects. Multivariate analysis showed a significant association of epsilon4 with female gender. Our results confirm the increased frequency of epsilon4 in both probable and possible LOAD, failing to show a similar trend in VD and AAMI.
在晚发型可能阿尔茨海默病（LOAD；n = 64）、早发型可能阿尔茨海默病（EOAD；n = 32）、可能阿尔茨海默病（pAD；n = 44）、血管性痴呆（VD；n = 12）、年龄相关性记忆障碍（AAMI；n = 15）患者以及40名年龄匹配的健康对照者中评估了载脂蛋白E（Apo - E）基因型和等位基因频率。通过用限制性内切酶HhaI消化的聚合酶链反应产物来检测APO - E。与其他组相比，LOAD组中ε4频率有统计学意义的增加，与对照组相比，pAD组中ε4频率也有增加，而VD组和AAMI组与对照受试者相比未发现任何差异。多变量分析显示ε4与女性性别显著相关。我们的结果证实了在可能和疑似LOAD中ε4频率增加，而在VD和AAMI中未显示出类似趋势。
