Jin Y S, Anderson G, Mintz P D
Department of Pathology, University of Virginia Health Sciences Center, Charlottesville 22908, USA.
Transfusion. 1997 Aug;37(8):804-8. doi: 10.1046/j.1537-2995.1997.37897424402.x.
Transfusion-associated graft-versus-host disease can be prevented by gamma irradiation of blood components. Red cells (RBCs) from sickle cell disease patients may exhibit oxidative changes of RBC membranes due to the instability of hemoglobin (Hb) S. Persons with sickle cell trait are eligible to donate blood, and 35 to 45 percent of their total Hb is Hb S. The effect of gamma irradiation on RBCs from such persons is of interest.
RBCs from 12 donors with sickle cell trait (Hb AS) and from 12 with normal Hb (Hb AA) were studied. Each of the 24 RBC units was divided equally into two transfer bags via a sterile connecting device. One bag from each RBC unit received a 2500-cGy dose of gamma irradiation at its mid-plane and was stored at 4 degrees C; the second set of bags was stored without irradiation. For RBCs from 6 donors with Hb AS and 6 donors with Hb AA, units were irradiated on Day 7 and studied on Day 35 of storage (Group 1). For the RBCs from the other 6 donors with Hb AS and the other 6 donors with Hb AA, units were irradiated on Day 28 and studied on Day 42 of storage (Group 2).
For Group 1 and Group 2, plasma potassium and plasma Hb concentrations were significantly higher and RBC ATP concentrations were slightly lower in the irradiated units than in the nonirradiated units. In Group 1 and Group 2, there were no significant differences in the plasma potassium or RBC ATP concentrations in either the irradiated or the nonirradiated units of RBCs from donors with Hb AS and donors with Hb AA. Plasma Hb concentrations were consistently lower in the units from donors with Hb AS, whether or not they were irradiated. However, in both groups, proportionally similar changes in plasma Hb concentration were detected when the irradiated Hb AS and Hb AA units were compared to nonirradiated Hb AS and Hb AA units.
Gamma irradiation of RBCs from donors with Hb AS or with Hb AA resulted in comparable changes in plasma potassium, RBC ATP, and plasma Hb concentrations, although donors with Hb AS had lower plasma Hb. RBCs from donors with Hb AS subjected to 2500 cGy of gamma irradiation did not evidence a storage lesion greater than that seen in RBCs from donors with Hb AA.
输血相关移植物抗宿主病可通过对血液成分进行γ射线照射来预防。镰状细胞病患者的红细胞（RBC）可能由于血红蛋白（Hb）S的不稳定性而表现出红细胞膜的氧化变化。具有镰状细胞性状的人有资格献血，其总Hb的35%至45%为Hb S。γ射线照射对此类人的红细胞的影响令人关注。
研究了12名具有镰状细胞性状（Hb AS）的献血者和12名具有正常Hb（Hb AA）的献血者的红细胞。通过无菌连接装置将24个红细胞单位中的每一个均分成两个转移袋。每个红细胞单位的一个袋子在其中心平面接受2500 cGy剂量的γ射线照射，并储存在4℃；第二组袋子未照射直接储存。对于6名具有Hb AS的献血者和6名具有Hb AA的献血者的红细胞，单位在储存第7天进行照射，并在储存第35天进行研究（第1组）。对于另外6名具有Hb AS的献血者和另外6名具有Hb AA的献血者的红细胞，单位在储存第28天进行照射，并在储存第42天进行研究（第2组）。
对于第1组和第2组，照射后的单位血浆钾和血浆Hb浓度显著更高，红细胞ATP浓度略低于未照射的单位。在第1组和第2组中，具有Hb AS的献血者和具有Hb AA的献血者的红细胞照射或未照射单位的血浆钾或红细胞ATP浓度均无显著差异。无论是否照射，具有Hb AS的献血者的单位血浆Hb浓度始终较低。然而，在两组中，当将照射的Hb AS和Hb AA单位与未照射的Hb AS和Hb AA单位进行比较时，血浆Hb浓度检测到成比例的相似变化。
对具有Hb AS或Hb AA的献血者的红细胞进行γ射线照射导致血浆钾、红细胞ATP和血浆Hb浓度发生类似变化，尽管具有Hb AS的献血者血浆Hb较低。接受2500 cGyγ射线照射的具有Hb AS的献血者的红细胞未显示出比具有Hb AA的献血者的红细胞更大的储存损伤。
