核内不均一RNA中的5'帽结构：缺乏m32,2,7G以及带帽分子的大小分布

5' Caps in hnRNA: absence of m32,2,7G and size distribution of capped molecules.

作者信息

Fernandez-Munoz R, Lavi U, Darnell J E

出版信息

Nucleic Acids Res. 1977 Oct;4(10):3357-69. doi: 10.1093/nar/4.10.3357.

DOI:10.1093/nar/4.10.3357

PMID:928063

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342658/

Abstract

In HeLa cells the "small nuclear" RNA has a cap II 5' structure (8)-- m32,2,7G(5') pppXmpYmp-- where X and Y are 2'0 methylated adenosine and uridine. In contrast hnRNA contains only cap I structures were the 2'0 methylated residue may be any base as was earlier reported for cytoplasmic mRNA (8,9,11). With a clear distinction between the source of these two caps an analysis of the size distribution of capped hnRNA could be performed which revealed over 65% of the capped hnRNA molecules were larger than cytoplasmic mRNA.

摘要

在海拉细胞中，“小核”RNA具有帽II 5'结构（8）——m32,2,7G(5') pppXmpYmp——其中X和Y分别是2'-O-甲基化腺苷和尿苷。相比之下，核内不均一RNA仅含有帽I结构，其中2'-O-甲基化残基可以是任何碱基，这与之前关于细胞质mRNA的报道一致（8,9,11）。由于这两种帽的来源有明显区别，因此可以对加帽核内不均一RNA的大小分布进行分析，结果显示超过65%的加帽核内不均一RNA分子比细胞质mRNA大。