海拉细胞异质核RNA中一个小转录多聚腺苷酸序列的特性

Properties of a small transcribed poly A sequence in heterogeneous nuclear RNA of HeLa cells.

作者信息

Venkatesan S, Nakazato H, Kopp D W, Edmonds M

出版信息

Nucleic Acids Res. 1979 Mar;6(3):1097-110. doi: 10.1093/nar/6.3.1097.

DOI:10.1093/nar/6.3.1097

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327756/

Abstract

A class of heterogeneous nuclear RNA (hnRNA) molecules contain an internal transcribed poly A sequence of close to 25 uninterrupted AMP residues. HnRNA molecules containing this sequence are separable from those containing the large 3' terminal poly A sequence on the basis of their differential affinity for oligo dT cellulose. The fact that the transcribed small poly A and the 3' terminal poly A are not found in the same hnRNA molecules even though both are present in similar size classes and that the small poly A is absent from cytoplasmic messenger RNA (mRNA) has led us to propose a scheme for mRNA processing in which the 3' end of the small poly A in hnRNA becomes a priming size for the post-transcriptional addiction of the large poly A.

摘要

一类不均一核RNA（hnRNA）分子含有一段内部转录的多聚腺苷酸序列，该序列由接近25个不间断的AMP残基组成。含有此序列的hnRNA分子可根据其对寡聚dT纤维素的不同亲和力，与含有大的3'末端多聚腺苷酸序列的分子分离。尽管转录的小多聚腺苷酸和3'末端多聚腺苷酸存在于相似大小的类别中，但它们不会出现在同一hnRNA分子中，并且细胞质信使RNA（mRNA）中不存在小多聚腺苷酸，这一事实促使我们提出一种mRNA加工方案，即hnRNA中小多聚腺苷酸的3'末端成为大的多聚腺苷酸转录后添加的起始大小。

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