甲基对硫磷从市售制剂经人体皮肤的体外经皮渗透
In vitro percutaneous penetration of methyl-parathion from a commercial formulation through the human skin.
作者信息
Sartorelli P, Aprea C, Bussani R, Novelli M T, Orsi D, Sciarra G
机构信息
Institute of Occupational Medicine, University of Siena, Italy.
出版信息
Occup Environ Med. 1997 Jul;54(7):524-5. doi: 10.1136/oem.54.7.524.
OBJECTIVE
To compare in vitro percutaneous absorption of methyl-parathion dissolved in an acetone vehicle and in the form of a commercial formulation.
METHODS
Penetration through the human skin was measured in Franz diffusion cells with full thickness skin from a human cadaver as the membrane. The two tailed non-parametric Mann-Whitney U test was used to compare the cumulative diffusion of methyl-parathion in the receptor fluid of the cells at various time intervals.
RESULTS
In vitro skin penetration of methyl-parathion was significantly higher with the commercial formulation. The percentage of the applied dose absorbed after 24 hours was 5.20% v 1.35%. The mean lag time was < 8 hours.
CONCLUSION
Assessments of uptake and internal dose after exposure to pesticides should be based on the commercial products rather than active ingredients, because of the crucial role of the vehicle, as shown in this study.
目的
比较溶于丙酮载体中的甲基对硫磷与市售制剂形式的甲基对硫磷的体外经皮吸收情况。
方法
在Franz扩散池中，以取自人体尸体的全层皮肤作为膜，测量其对甲基对硫磷的渗透情况。采用双侧非参数曼-惠特尼U检验，比较不同时间间隔下细胞接受液中甲基对硫磷的累积扩散量。
结果
市售制剂的甲基对硫磷体外皮肤渗透显著更高。24小时后吸收的给药剂量百分比分别为5.20%和1.35%。平均滞后时间小于8小时。
结论
如本研究所示，由于载体的关键作用，接触农药后对吸收量和体内剂量的评估应基于市售产品而非活性成分。
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