Akashi H, Kaku Y, Kong X, Pang H
National Institute of Animal Health, Ibaraki, Japan.
Virus Res. 1997 Aug;50(2):205-13. doi: 10.1016/s0168-1702(97)00071-3.
The antigenicity and RNA genome structures of five Simbu serogroup bunyaviruses isolated in Japan and Australia were analyzed using monoclonal antibodies (Mabs) raised to Akabane (AKA) virus and oligonucleotide fingerprinting. The virion surface glycoprotein (G1) and the nucleocapsid (N) protein of heterologous viruses showed no reactivity to the Mabs, while the AKA-derived anti-G1 Mab (2F1) reacted with Peaton virus and all three AKA anti-N Mabs reacted with Tinaroo (TIN) virus at almost the same antibody titers as the homologous virus. Oligonucleotide fingerprinting analyses indicated that the three RNA species of all the viruses were unique and distinguishable. However, AKA and TIN viruses exhibited very similar S RNA oligonucleotide fingerprints, while the L and M RNA fingerprints were quite different. The S RNA sequence of TIN virus has been determined and compared with that of AKA and Aino viruses. The results revealed 95.1% S sequence homology between the AKA and TIN viruses. The antigenic and genetic comparisons of AKA and TIN viruses suggest that the two viruses may represent naturally occurring reassortant viruses.
利用针对赤羽（AKA）病毒制备的单克隆抗体（Mabs）和寡核苷酸指纹图谱，对在日本和澳大利亚分离出的5种辛布血清群布尼亚病毒的抗原性和RNA基因组结构进行了分析。异源病毒的病毒粒子表面糖蛋白（G1）和核衣壳（N）蛋白对这些单克隆抗体无反应，而源自AKA的抗G1单克隆抗体（2F1）与皮顿病毒发生反应，并且所有3种源自AKA的抗N单克隆抗体均与蒂纳鲁（TIN）病毒发生反应，其抗体效价与同源病毒几乎相同。寡核苷酸指纹图谱分析表明，所有病毒的3种RNA种类都是独特且可区分的。然而，AKA和TIN病毒表现出非常相似的S RNA寡核苷酸指纹图谱，而L和M RNA指纹图谱则有很大差异。已确定了TIN病毒的S RNA序列，并将其与AKA和爱诺病毒的S RNA序列进行了比较。结果显示，AKA和TIN病毒之间的S序列同源性为95.1%。对AKA和TIN病毒的抗原性和基因比较表明，这两种病毒可能代表自然发生的重配病毒。
