Akashi H, Kaku Y, Kong X G, Pang H
National Institute of Animal Health, Ibaraki, Japan.
J Gen Virol. 1997 Nov;78 ( Pt 11):2847-51. doi: 10.1099/0022-1317-78-11-2847.
The nucleotide sequence of the small (S) RNA segment of Akabane (AKA) bunyavirus was determined. The segment is 858 nucleotides long and contains two overlapping open reading frames (ORFs), which encode the nucleocapsid (N) and nonstructural (NSs) proteins, consistent with other bunyaviruses. Comparisons with the Aino virus S RNA sequence indicated that there is 73.5% identity in nucleotide sequence. However, the sequence identity of the 5' non-coding region of the genomic RNA between these two viruses is only 55%. The N ORFs from 20 Japanese and 2 Australian isolates of AKA virus were sequenced and subjected to phylogenetic analysis. This suggested that AKA virus has evolved in multiple lineages. Twenty-three isolates were grouped into three major clusters, and the cluster which includes recent isolates was subdivided into two branches. Thus, phylogenetic analysis of the AKA virus N protein gene gives a greater insight into bunyavirus evolution.
确定了赤羽（AKA）布尼亚病毒小（S）RNA片段的核苷酸序列。该片段长858个核苷酸，包含两个重叠的开放阅读框（ORF），它们编码核衣壳（N）蛋白和非结构（NSs）蛋白，这与其他布尼亚病毒一致。与艾诺病毒S RNA序列的比较表明，核苷酸序列的同一性为73.5%。然而，这两种病毒之间基因组RNA 5'非编码区的序列同一性仅为55%。对来自20株日本和2株澳大利亚的AKA病毒分离株的N ORF进行了测序并进行了系统发育分析。这表明AKA病毒已在多个谱系中进化。23株分离株被分为三个主要簇，包括近期分离株的簇又被细分为两个分支。因此，对AKA病毒N蛋白基因的系统发育分析能更深入地了解布尼亚病毒的进化。
