Schönherr E, Lügering N, Stoll R, Domschke W, Kresse H
Institute of Physiological Chemistry and Pathobiochemistry and Dept. of Medicine B, University of Münster, Germany.
Scand J Gastroenterol. 1997 Aug;32(8):785-90. doi: 10.3109/00365529708996535.
The small interstitial proteoglycans decorin and biglycan have been shown to interact with various extracellular matrix molecules and with transforming growth factor-beta. These interactions are proposed to be important for tissue repair, as the former interactions may affect the diameter and spacing of collagen fibrils, and the latter interaction the proliferation and differentiation of cells embedded in the matrix. The aim of this study is to localize these proteoglycans in the stomach and to investigate their suitability as potential markers of extracellular matrix activity in gastric lesions.
Immunohistochemical techniques and in situ hybridization were used to study the phenotypic expression of these two proteoglycans in routinely processed specimens of human stomach tissue from 8 patients with gastric ulcer and 10 healthy control persons.
In normal gastric tissue, immunostaining for both proteoglycans was found in the interstitium, with a more pronounced staining in the pylorus region than in the corpus area. In addition, biglycan showed a strong staining of parietal cells. In specimens of healing gastric ulcers a larger deposition of decorin throughout scar tissue could be shown, and a higher expression of decorin was also found by in situ hybridization. Biglycan was only found at the edges of the lesions.
This study shows for the first time the presence of decorin and biglycan in human gastric mucosa. We also showed that these proteoglycans may be involved in the gastric ulcer healing processes.
已证实小分子间质蛋白聚糖核心蛋白聚糖和双糖链蛋白聚糖可与多种细胞外基质分子及转化生长因子-β相互作用。这些相互作用被认为对组织修复很重要，因为前者的相互作用可能影响胶原纤维的直径和间距，而后者的相互作用则影响嵌入基质中的细胞的增殖和分化。本研究的目的是在胃中定位这些蛋白聚糖，并研究它们作为胃病变中细胞外基质活性潜在标志物的适用性。
采用免疫组织化学技术和原位杂交技术，研究8例胃溃疡患者和10例健康对照者的人胃组织常规处理标本中这两种蛋白聚糖的表型表达。
在正常胃组织中，间质中可发现两种蛋白聚糖的免疫染色，幽门区的染色比胃体部更明显。此外，双糖链蛋白聚糖在壁细胞中显示出强染色。在愈合期胃溃疡标本中，可显示核心蛋白聚糖在整个瘢痕组织中有更大的沉积，原位杂交也发现核心蛋白聚糖的表达更高。双糖链蛋白聚糖仅在病变边缘发现。
本研究首次表明人胃黏膜中存在核心蛋白聚糖和双糖链蛋白聚糖。我们还表明，这些蛋白聚糖可能参与胃溃疡愈合过程。
