McClure R D, Nunes L, Tom R
Department of Urology, University of California, San Francisco.
Fertil Steril. 1989 May;51(5):874-7. doi: 10.1016/s0015-0282(16)60683-0.
The authors compared a simple, two-layer Percoll density gradient technique with the swim-up technique for semen preparation in 128 men. In samples from normospermic (n = 55), oligospermic (n = 26), and asthenospermic (n = 29) men, the Percoll technique significantly improved yield, percent motility, and absolute number of motile sperm recovered, but in samples from oligoasthenospermic men (n = 18), only percent motility was improved. The Percoll density gradient also selected sperm with markedly improved function as assessed by both the sperm penetration assay and the fertility index. In 37 samples negative on the sperm penetration assay when processed with the swim-up technique, 19 (51%) became positive when processed with the Percoll technique. The Percoll density gradient is an improved method for semen manipulation as it allows greater recovery of sperm with higher motility and improved sperm function.
作者对128名男性采用简单的两层Percoll密度梯度技术与上游法进行精液制备。在正常精子症（n = 55）、少精子症（n = 26）和弱精子症（n = 29）男性的样本中，Percoll技术显著提高了回收率、活动率百分比以及回收的活动精子绝对数量，但在少弱精子症男性（n = 18）的样本中，仅活动率百分比得到了提高。通过精子穿透试验和受精指数评估，Percoll密度梯度还筛选出功能明显改善的精子。在用上游法处理时精子穿透试验呈阴性的37个样本中，用Percoll技术处理时有19个（51%）变为阳性。Percoll密度梯度是一种改进的精液处理方法，因为它能使具有更高活动率和改善精子功能的精子得到更大程度的回收。
