Rauscher A M, Fairweather-Tait S J, Wilson P D, Gorrick S, Greenwood R
Institute of Food Research, Norwich Laboratory, Colney, UK.
J Trace Elem Med Biol. 1997 Jun;11(2):65-70. doi: 10.1016/S0946-672X(97)80028-4.
Low plasma zinc concentrations and increased zinc excretion have been reported in patients with non-insulin dependent diabetes mellitus (NIDDM). This study was designed to compare zinc metabolism in ten NIDDM patients with ten healthy controls, using stable isotopes of zinc. Zinc absorption from a standard breakfast labelled extrinsically with 3 mg 67Zn was measured using a fecal monitoring technique and thermal ionization quadrupole mass spectrometry. One hour after the test meal each volunteer was given 0.5 mg 70Zn (as citrate) intravenously and blood samples taken at intervals for up to 6 days. Isotopic enrichment was measured and the data entered into a simple kinetic model to estimate the size and rate of turnover of exchangeable pools of zinc. Urinary zinc excretion was higher in the male diabetics than controls (p < 0.05), but not in females. Since the efficiency of absorption and endogenous losses of zinc were very variable between individuals, and there was only a limited amount of data, it was not possible to draw conclusion about the effect of NIDDM on zinc absorption and excretion. However, the fact that there were no differences in exchangeable zinc pool sizes suggests that zinc metabolism is not altered in NIDDM.
据报道，非胰岛素依赖型糖尿病（NIDDM）患者血浆锌浓度较低且锌排泄增加。本研究旨在使用锌的稳定同位素，比较10名NIDDM患者与10名健康对照者的锌代谢情况。采用粪便监测技术和热电离四极质谱法，测量从外源性标记有3毫克67Zn的标准早餐中吸收的锌。在测试餐后1小时，给每位志愿者静脉注射0.5毫克70Zn（柠檬酸盐形式），并在长达6天的时间内间隔采集血样。测量同位素富集情况，并将数据输入一个简单的动力学模型，以估计锌可交换池的大小和周转率。男性糖尿病患者的尿锌排泄量高于对照组（p < 0.05），但女性患者则不然。由于个体之间锌的吸收效率和内源性损失差异很大，且数据量有限，因此无法得出NIDDM对锌吸收和排泄影响的结论。然而，可交换锌池大小没有差异这一事实表明，NIDDM患者的锌代谢没有改变。
