Sánchez L, Santamaria P
Centro de Investigaciones Biológicas, Velázquez, Madrid, Spain.
Genetics. 1997 Sep;147(1):231-42. doi: 10.1093/genetics/147.1.231.
This article reports the breaking of ethological barriers through the constitution of soma-germ line chimeras between species of the melanogaster subgroup of Drosophila, which are ethologically isolated. Female Drosophila yakuba and D. teissieri germ cells in a D. melanogaster ovary produced functional oocytes that, when fertilized by D. melanogaster sperm, gave rise to sterile yakuba-melanogaster and teissieri-melanogaster male and female hybrids. However, the erecta-melanogaster and orena-melanogaster hybrids were lethal, since female D. erecta and D. orena germ cells in a D. melanogaster ovary failed to form oocytes with the capacity to develop normally. This failure appears to be caused by an altered interaction between the melanogaster soma and the erecta and orena germ lines. Germ cells of D. teissieri and D. orena in a D. melanogaster testis produced motile sperm that was not stored in D. melanogaster females. This might be due to incompatibility between the teissieri and orena sperm and the melanogaster seminal fluid. A morphological analysis of the terminalia of yakuba-melanogaster and teissieri-melanogaster hybrids was performed. The effect on the terminalia of teissieri-melanogaster hybrids of a mutation in doublesex, a regulatory gene that controls the development of the terminalia, was also investigated.
本文报道了通过构建黑腹果蝇亚组中在行为学上相互隔离的物种间的体-生殖系嵌合体来打破行为学屏障。在黑腹果蝇卵巢中的雅库布果蝇（Drosophila yakuba）和泰氏果蝇（D. teissieri）的雌性生殖细胞产生了功能性卵母细胞，这些卵母细胞在与黑腹果蝇精子受精后，产生了不育的雅库布-黑腹果蝇和泰氏-黑腹果蝇雌雄杂种。然而，艾氏-黑腹果蝇和奥氏-黑腹果蝇杂种是致死的，因为在黑腹果蝇卵巢中的艾氏果蝇（D. erecta）和奥氏果蝇（D. orena）雌性生殖细胞未能形成具有正常发育能力的卵母细胞。这种失败似乎是由黑腹果蝇体细胞与艾氏果蝇和奥氏果蝇生殖系之间改变的相互作用引起的。在黑腹果蝇睾丸中的泰氏果蝇和奥氏果蝇的生殖细胞产生了可游动的精子，但这些精子并未储存在黑腹果蝇雌性体内。这可能是由于泰氏果蝇和奥氏果蝇精子与黑腹果蝇精液之间的不相容性。对雅库布-黑腹果蝇和泰氏-黑腹果蝇杂种的尾器进行了形态学分析。还研究了双性基因（doublesex）（一种控制尾器发育的调节基因）的突变对泰氏-黑腹果蝇杂种尾器的影响。
