Bouquet de Jolinière J, Validire P, Canis M, Doussau M, Levardon M, Gogusev J
Service de Gynécologie-Obstétrique, Hôpital Beaujon, Clichy, France.
Hum Reprod Update. 1997 Mar-Apr;3(2):117-23. doi: 10.1093/humupd/3.2.117.
A human epithelial-like cell line derived from peritoneal implants from a patient with gonadotrophin-releasing hormone (GnRH) agonist-resistant endometriosis graded as stage IVd according to the American Fertility Society classification was established in vitro. This cell line, designated FbEM-1, exhibited an epithelial-like morphology, grew in suspension and was immunoreactive for cytokeratins 8, 18, 19, vimentin and human leukocyte class I antigens. The cultured cells were negative for various haematopoietic cell markers, including the lymphoid cell antigens CD3, CD20 and CD45, von Willebrandt factor, carcinoembryonic antigen and the carcinoma antigen-125 (CA-125). In addition, the FbEM-1 cells were found to be moderately positive for periodic acid Schiff's (PAS) solution but were negative for alpha-naphthyl acetate esterase, peroxidase and chloroacetate esterase activities. Using specific antibodies against the progesterone, androgen and oestrogen receptors, approximately 40% and 5-10% of the cells immunostained for progesterone and androgen receptors respectively, while oestrogen receptors were not detected. On cytogenetic analysis using R-banding, these cells showed numerous chromosomal aberrations, including loss of one chromosome X, 4q+, 5q+, trisomy 7,8 and 10 and tetrasomy of chromosomes 17, 18, 19 and 20. These data show that the continuously growing FbEM-1 cell line established from endometriotic implants may be useful in achieving better understanding of the histogenesis of endometriosis.
根据美国生育协会的分类，从一名患有促性腺激素释放激素（GnRH）激动剂抵抗性Ⅳd期子宫内膜异位症患者的腹膜植入物中分离出一种人上皮样细胞系，并在体外建立了该细胞系。这个细胞系命名为FbEM-1，呈现上皮样形态，悬浮生长，对细胞角蛋白8、18、19、波形蛋白和人类白细胞I类抗原具有免疫反应性。培养的细胞对各种造血细胞标志物呈阴性，包括淋巴细胞抗原CD3、CD20和CD45、血管性血友病因子、癌胚抗原和癌抗原125（CA-125）。此外，发现FbEM-1细胞对过碘酸希夫（PAS）溶液呈中度阳性，但对α-萘乙酸酯酶、过氧化物酶和氯乙酸酯酶活性呈阴性。使用针对孕酮、雄激素和雌激素受体的特异性抗体，分别约有40%和5%-10%的细胞对孕酮和雄激素受体进行免疫染色，而未检测到雌激素受体。使用R显带进行细胞遗传学分析时，这些细胞显示出许多染色体畸变，包括一条X染色体缺失、4q+、5q+、7、8和10三体以及17、18、19和20号染色体四体。这些数据表明，从子宫内膜异位植入物建立的持续生长的FbEM-1细胞系可能有助于更好地理解子宫内膜异位症的组织发生。
