Turcotte R
Rev Can Biol. 1977 Sep;36(3):253-63.
Intraperitoneal inoculation of CF1 mice with Bacillus Calmette-Guérin (BCG) protected many of them against the ascites form of Ehrlich carcinoma; and, for those that developed cancer, complete regression occurred in up to 50% of the cases at an advanced state of the neoplastic disease. In contrast, when a booster dose of BCG was administered in admixture with tumor cells, the incidence of the tumor was lower and tumor regressions were very rarely observed in mice that developed cancer. Trypan blue, an inhibitor of lysosomal enzymes of macrophages, was found to markedly suppress the natural (innate) antitumor resistance of control mice as well as the acquired resistance and tumor regressions of BCG-sensitized mice. Moreover, a comparison of the cytotoxic activity of the adherent (macrophages) and nonadherent (predominantly lymphocytes) cells isolated from the peritoneal cavity of BCG-sensitized mice, as measured by the inhibition of DNA synthesis, revealed that the effector cells were amongst the macrophages. In contrast, spleen macrophages were devoid of cytotoxicity. The spleen lymphocytes from both BCG-sensitized and control mice possessed about the same significant cytotoxic activity. These results indicate that the activated peritoneal macrophages, induced by a local injection of BCG, could play an important role in the antitumor immunity against Ehrlich carcinoma.
给CF1小鼠腹腔接种卡介苗（BCG）可保护许多小鼠免受艾氏腹水癌腹水型的侵害；对于那些患上癌症的小鼠，在肿瘤疾病晚期，高达50%的病例出现了完全消退。相比之下，当将一剂加强剂量的卡介苗与肿瘤细胞混合注射时，肿瘤发生率较低，且在患上癌症的小鼠中很少观察到肿瘤消退。台盼蓝是巨噬细胞溶酶体酶的抑制剂，发现它能显著抑制对照小鼠的天然（固有）抗肿瘤抵抗力以及卡介苗致敏小鼠的获得性抵抗力和肿瘤消退。此外，通过测量DNA合成抑制来比较从卡介苗致敏小鼠腹腔分离出的贴壁细胞（巨噬细胞）和非贴壁细胞（主要是淋巴细胞）的细胞毒性活性，发现效应细胞存在于巨噬细胞之中。相比之下，脾巨噬细胞没有细胞毒性。卡介苗致敏小鼠和对照小鼠的脾淋巴细胞具有大致相同的显著细胞毒性活性。这些结果表明，局部注射卡介苗诱导的活化腹腔巨噬细胞在针对艾氏癌的抗肿瘤免疫中可能发挥重要作用。
