人胎盘血浆息肉的体外形成及其通过“反向离心”法的分离（作者译）

[In-vitro formation of plasma polyps in the human placenta and their isolation by means of "inverted centrifugation" (author's transl)].

Mitterer K E, Thorn W, Ruhrmann K

Res Exp Med (Berl). 1977 Sep 30;171(2):155-62. doi: 10.1007/BF01851362.

Abstract

Histological observations on sliced human placenta shaked in Krebs-Ringer-solution showed an high plasma polyp formation by the syncytiotrophoblast without any monoidoacetate or sodiumfluoride poisoning [1]. 2. The high plasma polyp formation of placenta slices incubated with gased (96% O2, 4% CO2) Krebs-Ringer-solution and shaked at 37 degrees C decreased rapidly and lasted up to 40 min. 3. Perfusion of the placenta intervillosum with fetal serum added Krebs-Ringer-Solution gave results in plasma polyp production up to six times higher than serum free Krebs-Ringer-solution perfusions. 4. Plasma polyp isolation and concentration by one step is discribed applying the inverted centrifugation technique: Plasma polyps migrate centripetally in Krebs-Ringer-solution containing 6% Ficoll (Pharmacia) and were concentrated in the tip of a tube invertedly placed into a swing-out-rotor.

摘要

对在 Krebs-Ringer 溶液中振荡的人胎盘切片进行组织学观察发现，合体滋养层形成了大量血浆息肉，且未出现任何碘代乙酸或氟化钠中毒现象[1]。2. 用含气（96% O₂，4% CO₂）的 Krebs-Ringer 溶液孵育胎盘切片并在 37℃振荡，其血浆息肉的大量形成迅速减少，并持续长达 40 分钟。3. 用添加了胎牛血清的 Krebs-Ringer 溶液灌注胎盘绒毛间隙，产生的血浆息肉比无血清的 Krebs-Ringer 溶液灌注高出六倍。4. 描述了采用反向离心技术一步分离和浓缩血浆息肉的方法：血浆息肉在含有 6% 菲可（Pharmacia）的 Krebs-Ringer 溶液中向心迁移，并浓缩在倒置放入摆动式转子中的管尖处。