Radford A, Parish J H
Department of Biology, University of Leeds, United Kingdom.
Fungal Genet Biol. 1997 Jun;21(3):258-66. doi: 10.1006/fgbi.1997.0979.
Neurospora crassa is an organism with a 7-decade contribution to genetic research. in a genome of 42.9 Mb and just over 1000 map units, to date over 800 different genes have been identified by phenotype and/or map location, and 222 genes have been characterized by sequencing. Methods by which analysis of the genome has been carried out are discussed, including linkage, RFLP, and chromosome walking. Characterized centomeres, telomeres, the nucleolar organizer and the dispersed 5S rRNA genes are discussed. Analysis of the protein-encoding genes is undertaken, using new software for the querying of standard sequence databases. Gene analysis includes consensus sequences for transcription and RNA splicing and new insights into codon usage.
粗糙脉孢菌是一种对基因研究有长达70年贡献的生物体。在一个42.9兆碱基且略超过1000个图谱单位的基因组中，迄今为止，通过表型和/或图谱定位已鉴定出800多个不同的基因，并且通过测序对222个基因进行了表征。讨论了进行基因组分析的方法，包括连锁分析、限制性片段长度多态性分析和染色体步移。还讨论了已表征的着丝粒、端粒、核仁组织区和分散的5S核糖体RNA基因。利用用于查询标准序列数据库的新软件对蛋白质编码基因进行了分析。基因分析包括转录和RNA剪接的共有序列以及对密码子使用的新见解。
