粗糙脉孢菌的基因组分析；精氨酸-1（arg-1）、蛋氨酸-6（met-6）、未知-7（un-7）和核糖体产生-1（rip-1）四个基因座的克隆及相关染色体步移

Genome analysis in Neurospora crassa; cloning of four loci arginine-1 (arg-1), methionine-6 (met-6), unknown-7 (un-7), and ribosome production-1 (rip-1) and associated chromosome walking.

作者信息

Schmidhauser T J, Liu Y Z, Liu H, Zhou S

机构信息

Department of Biology, University of Southwestern Louisiana, Lafayette 70504-2451, USA.

出版信息

Fungal Genet Biol. 1997 Jun;21(3):323-8. doi: 10.1006/fgbi.1997.0974.

DOI:10.1006/fgbi.1997.0974

PMID:9290245

Abstract

We have cloned four Neurospora crassa genes by complementation analysis. Cloned genes include the arginine-1 (arg-1), methionine-6 (met-6), unknown-7 (un-7), and ribosome production-1 (rip-1) loci. Chromosome walks were initiated in ordered cosmid libraries from the cloned loci. A total of about 700 kb of the Neurospora genome is covered in these walks.

摘要

我们通过互补分析克隆了四个粗糙脉孢菌基因。克隆的基因包括精氨酸-1（arg-1）、蛋氨酸-6（met-6）、未知-7（un-7）和核糖体产生-1（rip-1）位点。从克隆位点开始，在有序黏粒文库中进行染色体步移。这些步移共覆盖了粗糙脉孢菌基因组约700 kb的区域。