粗糙脉孢菌中氮代谢物阻遏所需调控基因nit-2的分子克隆。
Molecular cloning of nit-2, a regulatory gene required for nitrogen metabolite repression in Neurospora crassa.
作者信息
Stewart V, Vollmer S J
出版信息
Gene. 1986;46(2-3):291-5. doi: 10.1016/0378-1119(86)90414-2.
PMID:2879771
Abstract
We used an efficient sib-selection procedure to isolate a cosmid clone that complemented a mutated nit-2 gene of Neurospora crassa. Restriction fragment length polymorphism mapping indicated that the cosmid DNA insert was derived from linkage group IL, between 5S rDNA locus 12 and mt, the region of the N. crassa genome that contains nit-2. We conclude that the cosmid carries the nit-2 gene.
摘要
我们使用了一种高效的同胞选择程序来分离一个黏粒克隆，该克隆可互补粗糙脉孢菌的一个突变的nit-2基因。限制性片段长度多态性图谱分析表明，黏粒DNA插入片段源自连锁群IL，位于5S rDNA基因座12和mt之间，即粗糙脉孢菌基因组中包含nit-2的区域。我们得出结论，该黏粒携带nit-2基因。
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