Fidel P L, Ginsburg K A, Cutright J L, Wolf N A, Leaman D, Dunlap K, Sobel J D
Department of Microbiology, Immunology, and Parasitology, Louisiana State University Medical Center, New Orleans 70112-1393, USA.
J Infect Dis. 1997 Sep;176(3):728-39. doi: 10.1086/514097.
Studies from women with recurrent vulvovaginal candidiasis (RVVC) and from an animal model of experimental vaginitis suggest that deficiencies in immune function should be examined at the local rather than systemic level. Evidence of vaginal cell-mediated immunity (CMI) was evaluated for the first time in cervicovaginal lavage (CVL) fluid from RVVC patients. Results showed that although constitutive Th1- and Th2-type cytokine expression was detectable in CVL fluid from normal women, and differences in cytokines were observed in RVVC patients, limitations in experimental design of such de novo analyses urged caution in interpretation. Alternatively, attempts were made to establish conditions in control subjects whereby vaginal immunity could be detected after intravaginal challenge with Candida antigen. Preliminary results showed that Th1-type cytokines (interleukin-2 and -12, interferon-gamma) and histamine were increased 16-18 h after intravaginal introduction of Candida skin test antigen. Intravaginal antigenic challenge represents a novel approach for studying Candida-specific vaginal CMI.
对复发性外阴阴道念珠菌病（RVVC）女性及实验性阴道炎动物模型的研究表明，免疫功能缺陷应在局部而非全身水平进行检查。首次对RVVC患者宫颈阴道灌洗液（CVL）中的阴道细胞介导免疫（CMI）证据进行了评估。结果显示，虽然在正常女性的CVL液中可检测到组成性的Th1型和Th2型细胞因子表达，且在RVVC患者中观察到细胞因子存在差异，但此类从头分析的实验设计存在局限性，因此在解释时需谨慎。另外，研究人员尝试在对照受试者中建立条件，以便在阴道内用念珠菌抗原激发后检测阴道免疫。初步结果显示，在阴道内引入念珠菌皮肤试验抗原后16 - 18小时，Th1型细胞因子（白细胞介素-2和-12、干扰素-γ）和组胺增加。阴道内抗原激发是研究念珠菌特异性阴道CMI的一种新方法。
