爱泼斯坦-巴尔病毒与肿瘤启动子协同诱导永生化人上皮细胞发生恶性转化。
Epstein-Barr virus in synergy with tumor-promoter-induced malignant transformation of immortalized human epithelial cells.
作者信息
Li B M, Ji Z W, Liu Z S, Zeng Y
机构信息
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing, P. R. China.
出版信息
J Cancer Res Clin Oncol. 1997;123(8):441-6. doi: 10.1007/BF01372548.
It is difficult to study how Epstein-Barr virus (EBV) causes transformation of human epithelial cells. The major difficulty is that cultured human epithelial cells do not express EBV receptor (complement receptor 2, CR2), hence EBV cannot infect such epithelial cells directly. In order to investigate the role of EBV in the transformation of human epithelial cells, pSG-CR2-Hyg carrier was transfected into immortalized human epithelial cells (293 cells) to express EBV receptor. EBV could infect these CR2-positive cells directly, and expressed EBV antigens. EBV-infected epithelial cells grew in piles with multiple cellular layers and lost contact inhibition in vitro. In soft-agar culture containing 12-0-tetradecanoylphorbol 13-acetate (TPA), EBV-infected 293 cells formed more and larger colonies. When EBV-infected 293 cells were transplanted subcutaneously into nude mice, and treated with TPA, poorly differentiated carcinoma was induced. These results suggest that EBV could induce the malignant transformation of immortalized human epithelial cells in synergy with TPA.
研究爱泼斯坦-巴尔病毒（EBV）如何导致人类上皮细胞转化是困难的。主要困难在于培养的人类上皮细胞不表达EBV受体（补体受体2，CR2），因此EBV不能直接感染此类上皮细胞。为了研究EBV在人类上皮细胞转化中的作用，将pSG-CR2-Hyg载体转染到永生化人类上皮细胞（293细胞）中以表达EBV受体。EBV可以直接感染这些CR2阳性细胞，并表达EBV抗原。EBV感染的上皮细胞在体外呈多层堆积生长，失去接触抑制。在含有12-0-十四烷酰佛波醇-13-乙酸酯（TPA）的软琼脂培养中，EBV感染的293细胞形成越来越大的集落。当将EBV感染的293细胞皮下移植到裸鼠中并用TPA处理时，可诱导出低分化癌。这些结果表明，EBV可与TPA协同诱导永生化人类上皮细胞发生恶性转化。
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