Fukushima T
Department of Otolaryngology, Kyoto Prefectural University of Medicine.
Nihon Jibiinkoka Gakkai Kaiho. 1997 Aug;100(8):870-9. doi: 10.3950/jibiinkoka.100.870.
The DNA ploidy patterns of fresh specimens from 145 patients with squamous cell carcinoma of the oral cavity and the pharynx were analyzed. The specimens were embedded in paraffin and the DNA ploidy patterns were analyzed mainly by flow cytometry. Aneuploid patterns were found in 70%, and they were found more frequently in hypopharyngeal carcinomas than in oral cavity carcinomas. Correlation between DNA ploidy and tumor size was statistically significant but metastasis to lymph nodes and clinical stage were not correlated. In stage I and II oral cavity carcinomas and stage III and IV epi and oropharyngeal carcinomas, survival was much poorer in the aneuploid than in the diploid group. Analysis of 30 patients showed better response to preoperative chemotherapy in the aneuploid than in the diploid group; the mean efficacy rates were as high as 68% in the aneuploid and 48% in the diploid group. Aneuploidy with a low DNA index did not respond well to chemotherapy compared with those with a high DNA index. DNA heterogeneity was found in 22% (4/18) of the oral cavity carcinomas and in 50% (6/ 12) of the hypopharyngeal carcinomas.
对145例口腔和咽鳞状细胞癌患者新鲜标本的DNA倍体模式进行了分析。标本用石蜡包埋，主要通过流式细胞术分析DNA倍体模式。发现70%的标本为非整倍体模式，下咽癌中出现非整倍体模式的频率高于口腔癌。DNA倍体与肿瘤大小之间的相关性具有统计学意义，但与淋巴结转移及临床分期无关。在I期和II期口腔癌以及III期和IV期下咽癌和口咽癌中，非整倍体组的生存率远低于二倍体组。对30例患者的分析显示，非整倍体组对术前化疗的反应优于二倍体组；非整倍体组的平均有效率高达68%，二倍体组为48%。与高DNA指数的非整倍体相比，低DNA指数的非整倍体对化疗反应不佳。在22%（4/18）的口腔癌和50%（6/12）的下咽癌中发现了DNA异质性。
