Angelsen A, Mecsei R, Sandvik A K, Waldum H L
Department of Physiology and Biomedical Engineering, Norwegian University of Technology and Science, Trondheim, Norway.
Prostate. 1997 Sep 15;33(1):18-25. doi: 10.1002/(sici)1097-0045(19970915)33:1<18::aid-pros4>3.0.co;2-5.
The neuroendocrine (NE) cells in the human prostate gland probably have a local regulatory role in both prostatic growth and differentiation as well as in the exocrine secretory process. Moreover, NE cells may be involved in the pathogenesis of both prostatic cancer and hyperplasia. To enhance the knowledge of the physiological and pathophysiological role of NE cells in the prostate gland, we wanted to establish an experimental animal model.
All lobes of the prostatic complex of rats with different serum levels of testosterone, as well as the prostate of the guinea pig, cat, and dog, were studied. Prostatic tissue fixed in different fixatives was studied with regard to NE cells by using cytochemical and immunohistochemical staining techniques, as well as Northern blotting and reverse transcriptase polymerase chain reaction (RT-PCR) for detection of rat chromogranin A (CgA) mRNA.
The present study indicates the absence of NE cells in the rat prostatic complex. No expression of CgA RNA was detectable either by Northern blotting or by RT-PCR. Only a few argyrophil cells in the prostatic complex of guinea pig were detected in tissue fixed in Bouin's solution.
Rat, guinea pig, cat, and dog are not suitable animals in physiological studies of NE cells in the prostate gland.
人类前列腺中的神经内分泌（NE）细胞可能在前列腺生长、分化以及外分泌分泌过程中发挥局部调节作用。此外，NE细胞可能参与前列腺癌和增生的发病机制。为了增进对NE细胞在前列腺中生理和病理生理作用的了解，我们希望建立一种实验动物模型。
研究了不同血清睾酮水平大鼠前列腺复合体的所有叶，以及豚鼠、猫和狗的前列腺。使用细胞化学和免疫组织化学染色技术，以及用于检测大鼠嗜铬粒蛋白A（CgA）mRNA的Northern印迹和逆转录聚合酶链反应（RT-PCR），对固定在不同固定剂中的前列腺组织进行NE细胞研究。
本研究表明大鼠前列腺复合体中不存在NE细胞。通过Northern印迹或RT-PCR均未检测到CgA RNA的表达。在Bouin溶液固定的组织中，仅在豚鼠前列腺复合体中检测到少数嗜银细胞。
大鼠、豚鼠、猫和狗不是前列腺NE细胞生理学研究的合适动物。
