Sankai T, Cho F, Yoshikawa Y
Tsukuba Primate Center for Medical Science, National Institute of Infectious Diseases, Ibaraki, Japan.
Am J Primatol. 1997;43(1):43-50. doi: 10.1002/(SICI)1098-2345(1997)43:1<43::AID-AJP3>3.0.CO;2-Y.
Ovaries of five adult female African green monkeys were stimulated by repeated administrations of equine chorionic gonadotrophin (eCG), followed by a single administration of human chorionic gonadotrophin (hCG). Oocytes were collected from enlarged follicles 28 h after hCG administration and incubated in vitro for 288 h. Oocytes that had extruded the first polar body were inseminated with spermatozoa that had been incubated for 4 to 6 h in medium with caffeine and dibutyryl cyclic AMP. Of these oocytes, 66% were fertilized and the incidence of polyspermy was 37%. Eighty-two percent of the fertilized eggs cleaved, with some developing into expanded blastocysts.
对五只成年雌性非洲绿猴的卵巢进行反复注射马绒毛膜促性腺激素（eCG）刺激，随后单次注射人绒毛膜促性腺激素（hCG）。在注射hCG后28小时从增大的卵泡中收集卵母细胞，并在体外培养288小时。将排出第一极体的卵母细胞与在含有咖啡因和二丁酰环磷酸腺苷的培养基中孵育4至6小时的精子进行受精。在这些卵母细胞中，66%受精，多精受精发生率为37%。82%的受精卵发生分裂，一些发育成扩张囊胚。
