Weber B A, Fagerholm P, Johansson B
St. Erik's Eye Hospital, Karolinska Institute, Stockholm, Sweden.
Cornea. 1997 Sep;16(5):560-3.
Endogenous hyaluronan (HA) has previously been shown to be a part of corneal wound healing after photorefractive keratectomy (PRK). HA is known to bind considerable amounts of water. This study aimed to show whether HA colocalized with water in the wound area after PRK.
Five New Zealand White rabbits were subjected to PRK in one eye each. After 4 weeks, the rabbits were killed and the corneas were freeze-sectioned. The freeze-dried sections were exposed to soft x-rays generated at 3 kV and the microradiographs were evaluated densitometrically. The same sections were then stained for the presence of HA using the HA-binding region of bovine nasal cartilage proteoglycans linked to biotin.
The microradiographs showed, in a zone underlying the epithelium, that the water content was increased. In the corresponding zone, the cornea stained positive for HA.
HA that is reactively formed in the corneal wound after PRK colocalized to the hydrated area of the corresponding location as revealed by quantitative microradiography. The findings suggest that HA causes local shifts in water content in the corneal wound and thereby also local shifts in transparency.
先前已表明内源性透明质酸（HA）是准分子激光原位角膜磨镶术（PRK）后角膜伤口愈合的一部分。已知HA能结合大量水分。本研究旨在表明PRK后伤口区域的HA是否与水共定位。
对5只新西兰白兔每只的一只眼睛进行PRK。4周后，处死兔子，将角膜冷冻切片。将冷冻干燥的切片暴露于3 kV产生的软X射线下，并对微射线照片进行密度测定评估。然后使用与生物素连接的牛鼻软骨蛋白聚糖的HA结合区域对相同切片进行HA染色。
微射线照片显示，在上皮下方的区域，水分含量增加。在相应区域，角膜HA染色呈阳性。
如定量微射线照相所示，PRK后角膜伤口中反应性形成的HA与相应位置的水合区域共定位。研究结果表明，HA导致角膜伤口中水分含量的局部变化，从而也导致透明度的局部变化。
