Weber B A, Fagerholm P P
St. Erik's Eye Hospital, Karolinska Institute, Stockholm, Sweden.
Acta Ophthalmol Scand. 1998 Oct;76(5):537-40. doi: 10.1034/j.1600-0420.1998.760505.x.
To compare the extension of the subepithelial low dry mass layer after plano and refractive keratectomy, in order to know more about the factors that influence the corneal wound healing response.
Thirty rabbits assigned into two groups were treated on one eye each with plano and refractive keratectomy, respectively. In each group five rabbits were sacrificed after 10 days, 4 weeks and 21 weeks, respectively. The corneal buttons were excised, freeze-sectioned and freeze-dried. On the freeze-dried sections microradiography was performed. In the treated eyes the superficial stroma showed qualitatively underneath the epithelium areas with low dry mass, whose extension was measured by a modified planimetry.
The difference between the extension of subepithelial low dry mass areas in the plano and the refractive keratectomy group was small but statistically significant at 4 weeks. Within the refractive group the extension of low dry mass was significantly higher at 4 weeks compared to 21 weeks.
A flat surface ablation may cause a less pronounced healing response when compared to a steep curve refractive treatment.
比较单纯角膜切除术和屈光性角膜切除术后上皮下低干质量层的扩展情况，以便更深入了解影响角膜伤口愈合反应的因素。
将30只兔子分为两组，分别对每只兔子的一只眼睛进行单纯角膜切除术和屈光性角膜切除术。每组分别在术后10天、4周和21周处死5只兔子。切除角膜纽扣，进行冰冻切片和冻干处理。在冻干切片上进行显微放射摄影。在接受治疗的眼睛中，浅层基质在定性上显示上皮下有低干质量区域，其扩展通过改良的平面测量法进行测量。
单纯角膜切除术组和屈光性角膜切除术组上皮下低干质量区域的扩展差异较小，但在4周时具有统计学意义。在屈光性手术组中，4周时低干质量的扩展明显高于21周时。
与陡峭曲线屈光治疗相比，平面表面消融可能引起不太明显的愈合反应。
