Linton D, Owen R J, Stanley J
Molecular Biology Unit, Central Public Health Laboratory, London.
Res Microbiol. 1996 Nov-Dec;147(9):707-18. doi: 10.1016/s0923-2508(97)85118-2.
The nucleotide sequences for the 16S ribosomal RNA gene were compared for 33 species comprising the epsilon subdivision of the Proteobacteria (genera: Campylobacter, Helicobacter, Arcobacter and Wolinella). Regions specific for the genus Campylobacter and for five Campylobacter species important in human and/or veterinary medicine were identified. From these regions, PCR primer pairs were designed for use in species-specific identification. Primer pairs were validated against strains representing all taxa of campylobacter-like organisms. They did not amplify products other than from their five target species (C. upsaliensis, C. helveticus, C. fetus, C. hyointestinalis and C. lari), and they generated amplicons of defined size from large numbers of strains of those species. A primer pair suitable for identification of the genus Campylobacter was also identified and validated. This generated amplicons from all species of Campylobacter as well as from unnamed groups known to be within the genus, but not from any species or unnamed strains of Helicobacter, Arcobacter or Wolinella.
对属于变形菌门ε亚群的33个物种（弯曲杆菌属、幽门螺杆菌属、弓形杆菌属和沃林氏菌属）的16S核糖体RNA基因的核苷酸序列进行了比较。鉴定出了弯曲杆菌属以及对人和/或兽医学重要的五个弯曲杆菌物种特有的区域。从这些区域设计了用于物种特异性鉴定的PCR引物对。针对代表弯曲杆菌样生物体所有分类群的菌株对引物对进行了验证。它们除了从其五个目标物种（乌普萨拉弯曲杆菌、瑞士弯曲杆菌、胎儿弯曲杆菌、猪肠弯曲杆菌和海鸥弯曲杆菌）中扩增产物外，不会扩增其他产物，并且它们从这些物种的大量菌株中产生了确定大小的扩增子。还鉴定并验证了适合鉴定弯曲杆菌属的引物对。这从所有弯曲杆菌物种以及已知属于该属的未命名群体中产生了扩增子，但未从幽门螺杆菌、弓形杆菌或沃林氏菌的任何物种或未命名菌株中产生扩增子。
