Das S, Shraga D, Gannon C, Lam T T, Feng S, Brunet L R, Telford S R, Barthold S W, Flavell R A, Fikrig E
Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520-8031, USA.
Res Microbiol. 1996 Nov-Dec;147(9):739-51. doi: 10.1016/s0923-2508(97)85121-2.
A Borrelia burgdorferi chromosomal gene encodes a 30-kDa antigen (P30) that has considerable homology with periplasmic substrate-binding proteins of Gram-negative bacteria, and is recognized by antibodies in sera from a subset of patients with Lyme disease and from B. burgdorferi-infected mice. The p30 gene is 801 nucleotides in length and P30 contains 267 amino acids, with a predicted molecular mass of 30 kDa. The P30 amino acid region 36-258 has homology to conserved domains of the oligopeptide permease A of Gram-negative bacteria. Immunofluorescence studies using murine anti-P30 serum suggest that P30 is on the outer surface of B. burgdorferi. P30 expression could be detected in representatives of all 3 subspecies of B. burgdorferi sensu lato, but not in all of the tested strains. Antibodies to P30 were detected in sera of 18 out of 82 patients (22%) with Lyme disease, including individuals with early- or late-stage infection. Although antibodies to P30 are present in the sera of C3H/HeN mice infected with B. burgdorferi for at least 90 days, immunization with recombinant P30 does not protect mice from infection. We conclude that P30 is a putative substrate-binding protein of B. burgdorferi and is immunologically recognized in human and murine Lyme borreliosis.
伯氏疏螺旋体的一个染色体基因编码一种30 kDa抗原（P30），该抗原与革兰氏阴性菌的周质底物结合蛋白具有相当高的同源性，并且能被一部分莱姆病患者血清以及感染伯氏疏螺旋体的小鼠血清中的抗体识别。p30基因长度为801个核苷酸，P30含有267个氨基酸，预测分子量为30 kDa。P30氨基酸区域36 - 258与革兰氏阴性菌寡肽通透酶A的保守结构域具有同源性。使用鼠抗P30血清进行的免疫荧光研究表明，P30位于伯氏疏螺旋体的外表面。在伯氏疏螺旋体狭义的所有3个亚种的代表菌株中都能检测到P30的表达，但并非在所有测试菌株中都能检测到。在82例莱姆病患者中的18例（22%）血清中检测到了抗P30抗体，包括早期或晚期感染的个体。虽然在感染伯氏疏螺旋体至少90天的C3H/HeN小鼠血清中存在抗P30抗体，但用重组P30免疫并不能保护小鼠免受感染。我们得出结论，P30是伯氏疏螺旋体的一种假定底物结合蛋白，并且在人类和小鼠莱姆病螺旋体病中能被免疫识别。
