Rieu-Lesme F, Dauga C, Morvan B, Bouvet O M, Grimont P A, Doré J
INRA, C.R. de Clermont-Ferrand-Theix, Saint-Genès-Champanelle, France.
Res Microbiol. 1996 Nov-Dec;147(9):753-64. doi: 10.1016/s0923-2508(97)85122-4.
Thirteen strains of a new acetogenic bacterium were isolated from the rumen contents of lambs, llamas and bisons. This paper is the first report of Gram-positive coccoid spore-forming bacteria occurring in chains and able to use H2 + CO2 as energy source and produce acetate from this gas mixture. One of them, chosen as the reference strain for its efficiency in utilizing H2/CO2 likely via the acetyl-CoA pathway, was characterized in detail. The G + C ratio of the DNA of the organism was 46.5 mol%. The temperature and pH optimum were 37 degrees-40 degrees C and 6.3-6.8, respectively. Numerous organic substrates including some o-methylate aromatic compounds were used heterotrophically. The full 16S rRNA gene sequence was determined. The phylogeny, physiology, morphology and numerous features described here are sufficiently different from those of any bacteria described today to justify the definition of a new species. The name "New acetogenic bacterium" is temporarily proposed, awaiting a future taxonomic revision of the genus Clostridium.
从羔羊、美洲驼和野牛的瘤胃内容物中分离出13株新型产乙酸细菌。本文首次报道了革兰氏阳性、呈链状排列且能形成芽孢的细菌，该细菌能够利用H2 + CO2作为能源，并从这种气体混合物中产生乙酸盐。其中一株因其可能通过乙酰辅酶A途径高效利用H2/CO2而被选为参考菌株，并对其进行了详细表征。该生物体DNA的G + C含量为46.5 mol%。最适温度和pH分别为37℃ - 40℃和6.3 - 6.8。许多有机底物，包括一些邻甲基化芳香化合物，都可被其异养利用。测定了完整的16S rRNA基因序列。这里描述的系统发育、生理学、形态学和众多特征与当今描述的任何细菌都有足够的差异，足以证明定义一个新物种是合理的。暂时提出“新型产乙酸细菌”这一名称，以待未来对梭菌属进行分类修订。
