海洋尖尾藻主要碱性核蛋白的分离及其在染色体上的定位

Isolation of the major basic nuclear protein and its localization on chromosomes of the dinoflagellate, Oxyrrhis marina.

作者信息

Kato K H, Moriyama A, Huitorel P, Cosson J, Cachon M, Sato H

机构信息

Institute of Natural Sciences, Nagoya City University, Japan.

出版信息

Biol Cell. 1997 Mar;89(1):43-52. doi: 10.1016/s0248-4900(99)80080-x.

DOI:10.1016/s0248-4900(99)80080-x

PMID:9297782

Abstract

Basic nuclear proteins were extracted from isolated nuclei of Oxyrrhis marina. The HPLC pattern of the extract showed a single major peak, which consisted of a major band with an apparent molecular mass of 23 kDa on an SDS-PAGE gel. We designated this protein as Np23 because of its apparent molecular mass. The amino acid composition of this protein revealed its extremely basic nature with a high lysine content. Polyclonal antibodies were raised against Np23. Immunofluorescence microscopy showed that Np23 was localized within the nucleus of dividing and non-dividing cells as well, and immuno-electron microscopy showed that the protein was localized only on chromosomes. These data established that Np23 is the major basic chromosome protein of Oxyrrhis marina.

摘要

从微小拟菱形藻分离出的细胞核中提取了碱性核蛋白。提取物的高效液相色谱图显示有一个单一的主峰，该主峰在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）凝胶上表现为一条表观分子量为23 kDa的主要条带。由于其表观分子量，我们将该蛋白命名为Np23。该蛋白的氨基酸组成显示其具有极高的碱性，赖氨酸含量很高。制备了针对Np23的多克隆抗体。免疫荧光显微镜检查表明，Np23在分裂细胞和非分裂细胞的细胞核中均有定位，免疫电子显微镜检查表明该蛋白仅定位在染色体上。这些数据表明Np23是微小拟菱形藻的主要碱性染色体蛋白。