Katsuoka F, Kawakami Y, Arai T, Imuta H, Fujiwara M, Kanma H, Yamashita K
Institute of Clinical Medicine, The University of Tsukuba, Ibaraki, 305, Japan.
Biochem Biophys Res Commun. 1997 Sep 18;238(2):512-6. doi: 10.1006/bbrc.1997.7263.
Messenger RNA of receptor for advanced glycation end products (RAGE) is abundantly expressed in the lung. However, cell types expressing RAGE mRNA in the lung have not been identified. In order to elucidate the function of RAGE in pulmonary tissue, we have identified a cell type expressing RAGE mRNA by in situ hybridization and compared its expression level of RAGE mRNA by RNA blot analysis of isolated cells. In situ hybridization revealed that RAGE mRNA was intensely and specifically visualized in alveolar epithelial type II (AT-II) cells, and weakly in alveolar macrophages. The expression of RAGE mRNA in the primary culture of AT-II cells was at a high level, but that in alveolar macrophages isolated from alveolar lavage was under the level of detection by RNA blot analysis. These results showed that RAGE mRNA is specifically expressed in AT-II cells, and suggested that RAGE makes a substantial contribution to the function of AT-II cells in the lung.
晚期糖基化终末产物受体（RAGE）的信使核糖核酸在肺中大量表达。然而，肺中表达RAGE信使核糖核酸的细胞类型尚未明确。为了阐明RAGE在肺组织中的功能，我们通过原位杂交鉴定了一种表达RAGE信使核糖核酸的细胞类型，并通过对分离细胞的RNA印迹分析比较了其RAGE信使核糖核酸的表达水平。原位杂交显示，RAGE信使核糖核酸在Ⅱ型肺泡上皮（AT-II）细胞中强烈且特异性地显现，在肺泡巨噬细胞中则较弱。AT-II细胞原代培养中RAGE信使核糖核酸的表达水平较高，但通过RNA印迹分析，从肺泡灌洗中分离出的肺泡巨噬细胞中的表达水平低于检测限。这些结果表明，RAGE信使核糖核酸在AT-II细胞中特异性表达，并提示RAGE对肺中AT-II细胞的功能有重要作用。
