Konstantinov P, Burdarov I, Savova S, Vasilev V M
Vet Med Nauki. 1977;14(5):24-30.
Experiments were carried out with a total of 140 guinea pigs to study the effect of a tissue emulsion of swine embryos on some of the general defense reactions, the cell and humoral immunity of the organism in the treatment with an intact and a killed (with 3 per cent formalin) vaccine produced with two strains of Salmonella abortus ovis. Investigated were the phagocytic activity of the lung macrophages and the immobile cells of the reticulo-endothelial system in the spleen and the liver, the protein spectrum, the blood serum level of properdine, and the histologic changes in the parenchymal organs of the test animals. It was found that the application of a tissue emulsion simultaneously with the injection of the animals with an anti-Salmonella vaccine raised the unspecific reactivity of the organism. This was expressed with the rise of the gammaglobulin and properdine levels of the blood serum and the change in the immunologic pattern of the organism, an increase in the phagocytic activity of lung macrophages and RES immobile cells from 16 to 25 per cent, and the titer of agglutinins--from 10 to 32 times. Highest agglutinin titers of the blood and most active phagocytic defense were reached with the use of a vaccine having a whole antigen, combined with a tissue emulsion.
用总共140只豚鼠进行实验，以研究猪胚胎组织乳剂对机体某些一般防御反应、细胞免疫和体液免疫的影响，这些豚鼠用两株绵羊流产沙门氏菌制成的完整疫苗和灭活（用3%福尔马林）疫苗进行治疗。研究了肺巨噬细胞以及脾脏和肝脏中网状内皮系统固定细胞的吞噬活性、蛋白质谱、血清备解素水平以及实验动物实质器官的组织学变化。结果发现，在给动物注射抗沙门氏菌疫苗的同时应用组织乳剂可提高机体的非特异性反应性。这表现为血清γ球蛋白和备解素水平升高、机体免疫模式改变、肺巨噬细胞和网状内皮系统固定细胞的吞噬活性从16%提高到25%以及凝集素效价提高10至32倍。使用具有全抗原的疫苗并结合组织乳剂时，血液中的凝集素效价最高，吞噬防御最活跃。
