Bernal J L, Nozal M J, Toribio L, Serna M L, Borrull F, Marcé R M, Pocurull E
Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid, Spain.
J Chromatogr A. 1997 Aug 22;778(1-2):321-8. doi: 10.1016/s0021-9673(97)00388-9.
The potential of supercritical fluid chromatography (SFC) coupled on-line to solid-phase extraction (SPE) with disks for determining sixteen different polycyclic aromatic hydrocarbons (PAHs) was assessed. A preliminary study of the chromatographic separation was conducted that led to the use of SPE coupled to SFC for improved detection limits. Disks of two different materials, i.e., C18 and polystyrene-divinylbenzene, were assayed in terms of the variables influencing the extraction step. C18 disks provided the best results, with detection limits ranging from 0.1 to 1.5 micrograms l-1. The ensuing method was applied to river and tap water with good repeatability and reproducibility, and no interference from the sample matrix.
评估了超临界流体色谱法（SFC）与圆盘固相萃取（SPE）在线联用测定16种不同多环芳烃（PAHs）的潜力。进行了色谱分离的初步研究，结果表明采用SPE与SFC联用可提高检测限。针对影响萃取步骤的变量，对两种不同材料（即C18和聚苯乙烯-二乙烯基苯）的圆盘进行了测定。C18圆盘给出了最佳结果，检测限在0.1至1.5微克/升范围内。随后将该方法应用于河水和自来水中，具有良好的重复性和再现性，且不受样品基质的干扰。
