Nozal M J, Bernal J L, Toribio L, Marinero P, Moral O, Manzanas L, Rodriguez E
Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid, Spain.
J Chromatogr A. 1997 Aug 22;778(1-2):347-53. doi: 10.1016/s0021-9673(97)00473-1.
A high-performance liquid chromatographic method to determine glutathione, cysteine and N-acetylcysteine in rabbit retina, vitreous and lens has been developed. The thiols are separated using a 25 x 0.46-cm octadecylsilane column with 0.5 M phosphate buffer, pH 3, as mobile phase. The detection, at 412 nm, involves a post-column derivatization with 5,5-dithiobis(2-nitrobenzoic acid) in presence of cationic micelles of hexadecyltrimethylammonium bromide that enhances the sensitivity. The detection limits are 0.21, 0.92 and 0.61 mumol/g wet sample for glutathione, cysteine and N-acetylcysteine, respectively.
已开发出一种高效液相色谱法，用于测定兔视网膜、玻璃体和晶状体中的谷胱甘肽、半胱氨酸和N - 乙酰半胱氨酸。使用一根25×0.46厘米的十八烷基硅烷柱，以pH值为3的0.5 M磷酸盐缓冲液作为流动相来分离硫醇。在412纳米处进行检测，该检测涉及在十六烷基三甲基溴化铵阳离子胶束存在下，用5,5 - 二硫代双（2 - 硝基苯甲酸）进行柱后衍生化，这提高了灵敏度。谷胱甘肽、半胱氨酸和N - 乙酰半胱氨酸的检测限分别为0.21、0.92和0.61微摩尔/克湿样品。
