顺序注射分析方法测定药物中的谷胱甘肽。
Sequential Injection Analysis Method for the Determination of Glutathione in Pharmaceuticals.
机构信息
Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Rudjera Boskovica 35, 21000 Split, Croatia.
Department of Chemistry, Faculty of Science and Education, University of Mostar, Matice hrvatske bb, 88000 Mostar, Bosnia and Herzegovina.
出版信息
Sensors (Basel). 2024 Aug 31;24(17):5677. doi: 10.3390/s24175677.
A sequential injection analysis method for the determination of glutathione (GSH) in pharmaceuticals has been developed. It is based on the reduction of the Cu(II)-neocuproine complex by GSH and the formation of an orange-yellow colored Cu(I)-neocuproine complex with maximum absorbance at 458 nm. Under optimal conditions the method is characterized by a linear calibration range of 6.0 × 10-8.0 × 10 mol L ( = 3270 - 0.0010; = 0.9983), limit of detection of 2.0 × 10 mol L, limit of quantification of 6.7 × 10 mol L, repeatability (expressed as relative standard deviation) of 3.8%, and sampling rate of 60 h. The newly developed method has been successfully applied to the determination of GSH in pharmaceutical samples with no statistically significant difference between the results obtained and those produced by the standard Pharmacopoeia method.
已开发出一种用于测定药物中谷胱甘肽（GSH）的顺序注射分析方法。它基于 GSH 还原 Cu(II)-邻菲啰啉络合物，并形成最大吸收波长为 458nm 的橙黄色 Cu(I)-邻菲啰啉络合物。在最佳条件下，该方法的线性校准范围为 6.0×10-8.0×10 mol L（=3270-0.0010；=0.9983），检测限为 2.0×10 mol L，定量限为 6.7×10 mol L，重复性（表示为相对标准偏差）为 3.8%，采样率为 60 h。新开发的方法已成功应用于药物样品中 GSH 的测定，所得结果与药典标准方法产生的结果之间无统计学差异。
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