Barbas C, Castro M, Bonet B, Viana M, Herrera E
Facultad de CC Experimentales y Técnicas, Universidad S. Pablo-CEU, Madrid, Spain.
J Chromatogr A. 1997 Aug 22;778(1-2):415-20. doi: 10.1016/s0021-9673(97)00416-0.
A high-performance liquid chromatographic (HPLC) method to determine vitamins A, Ap and E simultaneously was developed with direct extraction of vitamins from rat tissues with n-hexane and probe sonicating. The dry residue was redissolved in chloroform-methanol. Vitamins A and Ap were detected by UV-Vis and vitamin E by fluorescence. Vitamin K, used as internal standard, was detected both the UV-Vis and by fluorescence. Standards and samples were checked for linearity giving correlation coefficients that were higher than 0.99 in the concentration range of 3.1-9.4 for vitamin A, 8.2-24.7 for vitamin Ap and 0.6-1.7 nmol/g in the case of liver extracts and 0.5-3.0 nmol/g in the case of placenta. The inta-assay precision (R.S.D) varied between 1.48 and 7.25, whereas inter-assay precision was between 4.99 and 7.03. Recoveries ranged between 94 +/- 8 and 107 +/- 5%. Results from the application of this method to different rat tissues having wide range of vitamin content are presented.
建立了一种高效液相色谱（HPLC）法，可同时测定维生素A、视黄醛和维生素E。该方法通过正己烷直接从大鼠组织中提取维生素，并采用探头超声处理。干燥残渣用氯仿 - 甲醇重新溶解。维生素A和视黄醛通过紫外 - 可见检测，维生素E通过荧光检测。用作内标的维生素K通过紫外 - 可见和荧光两种方式检测。对标准品和样品进行线性检查，维生素A在3.1 - 9.4浓度范围内、维生素视黄醛在8.2 - 24.7浓度范围内以及肝提取物中维生素E在0.6 - 1.7 nmol/g、胎盘组织中维生素E在0.5 - 3.0 nmol/g浓度范围内的相关系数均高于0.99。批内精密度（相对标准偏差）在1.48至7.25之间，而批间精密度在4.99至7.03之间。回收率在94±8%至107±5%之间。本文展示了该方法应用于不同维生素含量范围的大鼠组织的结果。
