Institute of Biotechnology, Collegium of Natural Sciences, University of Rzeszow, 1 Pigoń St., 35-310 Rzeszów, Poland.
Molecules. 2023 Apr 13;28(8):3449. doi: 10.3390/molecules28083449.
A new analytical approach to the simultaneous identification and quantification of vitamins A and E in three representative matrices (Parmesan, spinach, and almonds) was developed. The analyses were based on high-performance liquid chromatography with UV-VIS/DAD detection. The procedure was optimized by a significant reduction in the weight of the tested products and quantities of reagents added during the saponification and extraction stages. A full method validation study was performed for retinol at two concentration levels (LOQ and 200 × LOQ), which showed satisfactory results, with recoveries ranging from 98.8 to 110.1%, and an average CV of 8.9%. Linearity was tested in the range of 1-500 µg/mL and showed the coefficient of determination R = 0.999. The satisfactory recovery and precision parameters were achieved for α-tocopherol (LOQ and 500 × LOQ) in the range of 70.6-143.2%, with a mean CV equal to 6.5%. The observed linearity for this analyte in the concentration range of 1.06-532.0 µg/mL was R = 0.999. The average extended uncertainties were estimated, using a top-down approach of 15.9% and 17.6% for vitamin E and A, respectively. Finally, the method was successfully applied to determine vitamins in 15 commercial samples.
建立了一种新的分析方法，用于同时鉴定和定量三种代表性基质（帕尔马干酪、菠菜和杏仁）中的维生素 A 和 E。分析基于高效液相色谱法，采用紫外可见/二极管阵列检测。通过显著减少测试产品的重量和皂化和提取阶段添加的试剂数量，对该方法进行了优化。对视黄醇在两个浓度水平（LOQ 和 200×LOQ）进行了全面的方法验证研究，结果令人满意，回收率在 98.8%至 110.1%之间，平均 CV 为 8.9%。在线性测试范围内为 1-500μg/mL，相关系数 R = 0.999。对于α-生育酚（LOQ 和 500×LOQ），在 70.6-143.2%的范围内，平均 CV 等于 6.5%，达到了令人满意的回收率和精密度参数。该分析物在 1.06-532.0μg/mL 的浓度范围内的线性观察 R = 0.999。使用自上而下的方法，分别估计了维生素 E 和 A 的平均扩展不确定度为 15.9%和 17.6%。最后，该方法成功应用于测定 15 个商业样品中的维生素。
