Fulton R J, McDade R L, Smith P L, Kienker L J, Kettman J R
Luminex Corporation, Austin, TX 78727, USA.
Clin Chem. 1997 Sep;43(9):1749-56.
The FlowMetrix System is a multiplexed data acquisition and analysis platform for flow cytometric analysis of microsphere-based assays that performs simultaneous measurement of up to 64 different analytes. The system consists of 64 distinct sets of fluorescent microspheres and a standard benchtop flow cytometer interfaced with a personal computer containing a digital signal processing board and Windows95-based software. Individual sets of microspheres can be modified with reactive components such as antigens, antibodies, or oligonucleotides, and then mixed to form a multiplexed assay set. The digital signal-processing hardware and Windows95-based software provide complete control of the flow cytometer and perform real-time data processing, allowing multiple independent reactions to be analyzed simultaneously. The system has been used to perform qualitative and quantitative immunoassays for multiple serum proteins in both capture and competitive inhibition assay formats. The system has also been used to perform DNA sequence analysis by multiplexed competitive hybridization with 16 different sequence-specific oligonucleotide probes.
FlowMetrix系统是一个用于基于微球分析的流式细胞术分析的多路复用数据采集和分析平台，可同时测量多达64种不同的分析物。该系统由64组不同的荧光微球和一台标准台式流式细胞仪组成，该流式细胞仪与一台装有数字信号处理板和基于Windows95软件的个人计算机相连。单个微球组可用抗原、抗体或寡核苷酸等反应性成分进行修饰，然后混合形成多路复用分析组。数字信号处理硬件和基于Windows95的软件可对流式细胞仪进行全面控制并进行实时数据处理，从而能够同时分析多个独立反应。该系统已用于以捕获和竞争性抑制分析形式对多种血清蛋白进行定性和定量免疫分析。该系统还已用于通过与16种不同的序列特异性寡核苷酸探针进行多路复用竞争性杂交来进行DNA序列分析。
