Marr K A, Foster A P, Lees P, Cunningham F M, Page C P
Department of Veterinary Basic Sciences, Royal Veterinary College, North Mymms, Hertfordshire.
Res Vet Sci. 1997 May-Jun;62(3):253-60. doi: 10.1016/s0034-5288(97)90200-9.
The effect of antigen challenge on the state of activation of peripheral blood neutrophils from horses with chronic obstructive pulmonary disease (COPD) has been determined by measuring neutrophil superoxide anion formation. Prior to a seven-hour antigen challenge superoxide anion production by neutrophils from asymptomatic horses with COPD and normal horses in response to platelet activating factor (PAF) (with and without cytochalasin B), serum treated zymosan (STZ) and phorbol myristate acetate (PMA) was similar. Agonist-induced superoxide production by neutrophils from symptomatic COPD and normal horses remained unchanged five and 24 hours after antigen challenge. Interestingly, however, superoxide production by neutrophils from symptomatic COPD horses was significantly increased 24 hours after antigen challenge in the control samples for each agonist (basal superoxide production), a five-fold increase being measured in the presence of cytochalasin B. There was a small increase in superoxide production by neutrophils from normal horses but this only reached significance in one set of control samples. The change in activation state of circulating neutrophils during antigen challenge may facilitate the lung neutrophilia and subsequent tissue damage which occur in COPD.
通过测量中性粒细胞超氧阴离子的形成，已确定抗原激发对患有慢性阻塞性肺疾病（COPD）马匹外周血中性粒细胞活化状态的影响。在为期7小时的抗原激发之前，患有COPD的无症状马匹和正常马匹的中性粒细胞对血小板活化因子（PAF）（加或不加细胞松弛素B）、血清处理的酵母聚糖（STZ）和佛波酯（PMA）的超氧阴离子产生情况相似。有症状的COPD马匹和正常马匹的中性粒细胞在抗原激发后5小时和24小时，激动剂诱导的超氧产生保持不变。然而，有趣的是，在每次激动剂的对照样本中（基础超氧产生），有症状的COPD马匹的中性粒细胞在抗原激发后24小时超氧产生显著增加，在存在细胞松弛素B的情况下测量到增加了五倍。正常马匹的中性粒细胞超氧产生有小幅增加，但仅在一组对照样本中达到显著水平。抗原激发期间循环中性粒细胞活化状态的变化可能会促进COPD中发生的肺嗜中性粒细胞增多和随后的组织损伤。
